Autor(es): Venturini, James [UNESP] ; Soares, Cléverson Teixeira ; Belone, Andréa de Faria Fernandes ; Barreto, Jaison Antonio ; Ura, Somei ; Lauris, José Roberto Pereira ; Vilani-Moreno, Fátima Regina
Data: 2022
Identificador Persistente: http://hdl.handle.net/11449/219653
Origem: Oasisbr
Made available in DSpace on 2022-04-28T18:56:47Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-03-01
Objective: We investigated the in vitro and skin lesions production of cytokines in non-treated borderline tuberculoid (BT) and borderline lepromatous (BL) patients. Patients and Methods: Seven untreated, non-reactional BT patients and 12 untreated, non-reactional BL patients were studied. Levels of the cytokines IFN-γ, IL-10, TGF-β1 and TNF-α were measured in supernantant of peripheral blood mononuclear cells (PBMC) cultures, stimulated with specific M. leprae antigen (sonicated and whole). The cytokines iNOS, IL-10 and TGF-β1 were detected by immunohistochemistry in skin biopsies. Results: BT patients produced higher levels of IFN-γ than BL patients; iNOS expression in skin lesions was also higher in BT patients. TGF-β1 was detected in more cells in BL patients; IL-10 expression was similar in both groups. There was a negative correlation between iNOS and TGF-β1 expression in skin biopsies, positive correlation between TGF-b1 in skin lesions and bacillary index, as well as positive correlation between iNOS detected in skin biopsies and PBMC IFN- γ production. Conclusions: The BT patients had a mainly a Th1-profile of cytokines in their skin lesions and BL patients had aTh2 profile. © Lepra.
Faculdade de Medicina de Botucatu UNESP, Botucatu, SP
Equipe Técnica de Patologia Instituto Lauro de Souza Lima, Bauru, SP
Serviço de Epidemiologia Instituto Lauro de Souza Lima, Bauru, SP
Divisão de Pesquisa e Ensino Instituto Lauro de Souza Lima, Bauru, SP
Faculdade de Odontologia de Bauru Universidade de São Paulo, Bauru, SP
Faculdade de Medicina de Botucatu UNESP, Botucatu, SP
