Author(s): Cortez, Adriana ; Souza, Vanessa [UNESP] ; Fernandes, Telma [UNESP] ; Megid, Jane [UNESP]
Date: 2022
Persistent ID: http://hdl.handle.net/11449/220559
Origin: Oasisbr
Subject(s): Canine distemper; Direct immunofluorescence; HnRT-PCR; Kappa index
Made available in DSpace on 2022-04-28T19:02:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-01-01
The diagnostic value of RT-PCR and hemi-nested RT-PCR (hnRT-PCR) was compared in brain samples of dogs presenting neurological signs compatible with canine distemper. Samples of central nervous system (CNS) were collected from 68 dogs and tested by direct immunofluorescence test (RFID) and, independent of the results, they were stored at -20°C for at least three years. They were submitted to the RT-PCR and hnRT-PCR techniques aiming to determine the gene responsible for the viral nucleoprotein decoding. Fifty-nine samples were positive for RIFD, 40 for RT-PCR (Kappa = 0.358) and 54 for hnRT-PCR (Kappa = 0.740). All nine RIFD negative samples were also negative for RT-PCR and hnRT-PCR. In spite of the storage duration and proper sample conditions, the estimated accordance between hnRT-PCR and RIFD demonstrated that hnRT-PCR technique can be applied in retrospective studies.
Universidade de Santo Amaro Medicina Veterinária
Universidade Estadual Paulista
Universidade Estadual Paulista
