Autor(es): Valões Cardoso, M. Luiza ; Ferreira, Paula B. ; Wanderley, Artur M. ; Torres, Rodrigo A. ; Gomes, Mauricio Talebi ; Teixeira, Rodrigo H. F. [UNESP] ; Duarte, José M. B. [UNESP] ; Garcia, José E.
Data: 2022
Identificador Persistente: http://hdl.handle.net/11449/221241
Origem: Oasisbr
Assunto(s): 16S mtDNA; Brachyteles arachnoides; Forensic genetics; Illegal hunting; Threatened species
Made available in DSpace on 2022-04-28T19:26:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-01
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco
To control illegal wildlife-product trade and protect endangered species of animals, unambiguous identification of the captured specimens is essential. Forensic genetic tools have contributed to identify animal species for conservation purposes promoting accurate results for informing public policies and management of the biodiversity. The southern muriqui (Brachyteles arachnoides) is the largest non-human primate of the Neotropical region and is critically endangered (IUCN Red List of Threatened Species), mainly due to the illegal hunting for bushmeat. In this study, we describe a molecular method using PCR/RFLP to differentiate between bushmeat of southern muriqui and the meat of the domestic animals most commonly consumed in Brazil (Bos taurus, Ovis aries, Capra hircus, and Sus scrofa). The method is based on the amplification and digestion with BanI restriction enzyme of the 16S mtDNA region. We also examine 16S mtDNA sequences of the southern muriqui and other 13 sympatric and parapatric wild species of mammals also hunted for bushmeat to examine whether homologies of the BanI restriction sites could lead to species misidentification. The results indicate the utility of this tool as it represents a simple and cost-effective method to differentiate southern muriqui samples from those of the examined domestic and wild sympatric and parapatric species. We hope this molecular tool will help public authorities in crime prevention, and enhance law reinforcement of illegal hunting of threatened animal species.
Centro Acadêmico de Vitória Universidade Federal de Pernambuco
Departamento de Zoologia Universidade Federal de Pernambuco
Departamento de Ciências Ambientais Universidade Federal de São Paulo, Campus Diadema
Parque Zoológico Municipal Quinzinho de Barros
Universidade Estadual Paulista “Júlio de Mesquita Filho”
Departamento de Zootecnia Universidade Estadual Paulista “Júlio de Mesquita Filho”
Universidade Estadual Paulista “Júlio de Mesquita Filho”
Departamento de Zootecnia Universidade Estadual Paulista “Júlio de Mesquita Filho”
