Author(s): Jacinavicius, Fernanda R. ; Pacheco, Ana Beatriz F. ; Chow, Fungyi ; Verissimo da Costa, Giovani C. ; Kalume, Dário Eluan ; Rigonato, Janaina ; Schmidt, Eder C. ; Sant'Anna, Célia L.
Date: 2022
Persistent ID: http://hdl.handle.net/11449/221301
Origin: Oasisbr
Subject(s): ITRAQ; Microcystin; Morphology; Physiology; Proteomics; Ultrastructure
Made available in DSpace on 2022-04-28T19:27:21Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-08-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
Microcystis aeruginosa causes severe problems in freshwater environments worldwide due to its capacity to form blooms and produce toxins. Natural populations contain a mixture of strains that vary in morphology, genotype and microcystin production. Beyond its toxic effect in animals, the possible role of microcystin in the metabolism of the producing cell is also a topic of interest. Recent studies pointed to a protective role against cellular oxidative stress. Since natural populations include microcystin producing and non-producing strains, the latter must rely on other ecophysiological traits to thrive. Here, we compared a toxic and a non-toxic strain of M. aeruginosa displaying different morphotypes and growth behaviors, aiming to explore the role of microcystin in an integrated way to the primary metabolism. The toxic strain formed large floating colonies (150–1850 μm) with densely aggregated cells, numerous gas vesicles and lipid inclusions and a typical irregular thylakoid arrangement. The non-toxic strain remained submerged, formed small colonies (< 100 μm) with a dense mucilage covering few sparse cells with rare gas vesicles and various types of thylakoids. It showed a higher abundance of carboxysomes, cyanophycin and polyphosphate granules and also greater amounts of pigments than the toxic strain. The antioxidant potential was higher for the toxic strain and it presented a higher expression of proteins related to photosynthesis, protein folding and cellular redox homeostasis. These traits of the toxic strain were compatible with growth under higher irradiance and microcystin can be a key factor to this adaptation. Growing submerged, the non-toxic strain showed preference for a lower light intensity and invested in intracellular reserves. This study illustrates different adaptive strategies of M. aeruginosa morphotypes that can be valuable in nature, enabling this species to widely exploit freshwater ecosystems.
Nucleus of Phycology Institute of Botany, Avenida Miguel Stéfano, 3687, Caixa Postal 4005
Faculty of Pharmaceutical Science University of São Paulo, Avenida Prof. Lineu Prestes, 580, Butantã
Biophysics Institute Federal University of Rio de Janeiro, Avenida Carlos Chagas Filho, 373, Cidade Universitária
Institute of Bioscience University of São Paulo, Rua do Matão, 303, Butantã
Universidade Federal Fluminense (UFF), Rua Dr. Silvio Henrique Braune, 22, Centro
Laboratório Interdisciplinar de Pesquisas Médicas Instituto Oswaldo Cruz (IOC) Fundação Oswaldo Cruz (FIOCRUZ), Avenida Brasil, 4365, Manguinhos
Department of Zoology and Botany Instituto de Biociências Letras e Ciências Exatas, Rua Cristóvão Colombo, 2265, Jardim Nazareth
Department of Cell Biology Embryology and Genetics Federal University of Santa Catarina, Rua Eng. Agronômico Andrei Cristian Ferreira, s/n
FAPESP: 2011/50267-8
CAPES: 2018/1.1065.9.1
