Autor(es): Katak, Ricardo M. ; Rocha, Elerson M. ; Oliveira, Juan C. ; Muniz, Veranilce A. ; Oliveira, Marta R. ; Ferreira, Francisco A. S. ; Silva, William R. ; Roque, Rosemary A. ; de Souza, Antonia Q. L. ; Souza-Neto, Jayme A. [UNESP] ; Terenius, Olle ; Marinotti, Osvaldo ; Tadei, Wanderli P.
Data: 2022
Identificador Persistente: http://hdl.handle.net/11449/221910
Origem: Oasisbr
Assunto(s): Amazonian microbiota; Bacillus; Biological control; Bioprospecting; Mosquito
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
The Aedes aegypti mosquito is the primary vector of Dengue, Chikungunya and Zika causing major problems for public health, which requires new strategies for its control, like the use of entomopathogenic microorganisms. In this study, bacteria from various Amazonian environments were isolated and tested for their pathogenicity to A. aegypti larvae. Following thermal shock to select sporulated Bacillus spp., 77 bacterial strains were isolated. Molecular identification per 16S RNA sequences revealed that the assembled strains contained several species of the genus Bacillus and one species each of Brevibacillus, Klebsiella, Serratia, Achromobacter and Brevundimonas. Among the isolated Bacillus sp. strains, 19 showed larvicidal activity against A. aegypti. Two strains of Brevibacillus halotolerans also displayed larvicidal activity. For the first time, larvicidal activity against A. aegypti was identified for a strain of Brevibacillus halotolerans. Supernatant and pellet fractions of bacterial cultures were tested separately for larvicidal activities. Eight strains contained isolated fractions resulting in at least 50% mortality when tested at a concentration of 5 mg/mL. Further studies are needed to characterize the active larvicidal metabolites produced by these microorganisms and define their mechanisms of action.
Programa Multi-Institucional de Pós-Graduação em Biotecnologia—PPGBIOTEC Universidade Federal do Amazonas—UFAM
Programa de Pós-Graduação em Biotecnologia e Recursos Naturais da Amazônia—PPGMBT Universidade do Estado do Amazonas—UEA
Programa de Pós-Graduação em Biodiversidade e Biotecnologia—PPGBIONORTE Universidade Federal do Amazonas—UFAM
Programa de Pós-Graduação em Ciências Biológicas (Entomologia)—PPGENT Instituto Nacional de Pesquisas da Amazônia—INPA
Laboratório de Controle Biológico e Biotecnologia da Malária e Dengue—LCBBMD Instituto Nacional de Pesquisas da Amazônia—INPA
Faculdade de Ciências Agrárias—FCA Universidade Federal do Amazonas—UFAM
School of Agricultural Sciences Department of Bioprocesses and Biotechnology Central Multi User Laboratory São Paulo State University—UNESP
Department of Cell and Molecular Biology Microbiology Uppsala University
MTEKPrime
School of Agricultural Sciences Department of Bioprocesses and Biotechnology Central Multi User Laboratory São Paulo State University—UNESP
FAPESP: 2013/11343-6
