Detalhes do Documento

Genotypic Trypanosoma cruzi distribution and parasite load differ ecotypically and according to parasite genotypes in Triatoma brasiliensis from endemic and outbreak areas in Northeastern Brazil

Autor(es): Valença-Barbosa, Carolina ; Finamore-Araujo, Paula ; Moreira, Otacilio C. ; Vergara-Meza, Jose Gabriel ; Alvarez, Marcus Vinicius Niz [UNESP] ; Nascimento, Juliana R. ; Borges-Veloso, André ; Viana, Maria Carolina ; Lilioso, Maurício ; Miguel, Danilo Ciccone ; Gadelha, Fernanda Ramos ; Teixeira, Marta Maria Geraldes ; Almeida, Carlos Eduardo

Data: 2022

Identificador Persistente: http://hdl.handle.net/11449/222052

Origem: Oasisbr

Assunto(s): Epidemiology; Genotyping Trypanosoma cruzi; Lineages, oral outbreaks; Triatomines

Descrição

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)

This study aimed to identify the Trypanosoma cruzi genotypes and their relationship with parasitic load in distinct geographic and ecotypic populations of Triatoma brasiliensis in two sites, including one where a Chagas disease (ChD) outbreak occurred in Rio Grande do Norte state, Brazil. Triatomine captures were performed in peridomestic and sylvatic ecotopes in two municipalities: Marcelino Vieira – affected by the outbreak; and Currais Novos – where high pressure of peridomestic triatomine infestation after insecticide spraying have been reported. The kDNA-PCR was used to select 124 T. cruzi positive triatomine samples, of which 117 were successfully genotyped by fluorescent fragment length barcoding (FFLB). Moreover, the T. cruzi load quantification was performed using a multiplex TaqMan qPCR. Our findings showed a clear ecotypic segregation between TcI and TcII harboured by T. brasiliensis (p<0.001). Although no genotypes were ecotypically exclusive, TcI was predominant in peridomestic ecotopes (86%). In general, T. brasiliensis from Rio Grande do Norte had a higher T. cruzi load varying from 3.94 to 7.66 x 106 T. cruzi per insect. Additionally, TcII (median value=299,504 T. cruzi/intestine unit equivalents) had more than twice (p=0.1) the parasite load of TcI (median value=149,077 T. cruzi/intestine unit equivalents), which can be attributed to a more ancient co-evolution with T. brasiliensis. The higher prevalence of TcII in the sylvatic T. brasiliensis (70%) could be associated with a more diversified source of bloodmeals for wild insect populations. Either TcI or TcII may have been responsible for the ChD outbreak that occurred in the city of Marcelino Vieira. On the other hand, a smaller portion of T. brasiliensis was infected by TcIII (3%) in the peridomicile, in addition to T. rangeli genotype A (1%), often found in mixed infections. Our results highlight the need of understanding the patterns of T. cruzi genotype´s development and circulation in insect vectors and reservoirs as a mode of tracking situations of epidemiologic importance, as the ChD outbreak recently recorded for Northeastern Brazil.

Instituto de Biologia Universidade de Campinas – UNICAMP

Grupo Triatomíneos Instituto René Rachou Fundação Oswaldo Cruz – Fiocruz

Laboratório de Biologia Molecular e Doenças Endêmicas Instituto Oswaldo Cruz/Fiocruz

Instituto de Ciências Biomédicas Universidade de São Paulo-USP

Instituto de Biotecnologia de Botucatu Universidade Estadual Paulista

Instituto de Química Universidade Federal Fluminense

Instituto de Biologia Universidade Federal da Bahia

Instituto de Biotecnologia de Botucatu Universidade Estadual Paulista

CAPES: 165756/2018-7

FAPESP: 2016/08176-9

FAPESP: 2017-19420-0

CAPES: 306357/2019-4