Autor(es):
Salomão, Rebecca ; Neto, Ivo Vieira de Sousa ; Ramos, Gracielle Vieira ; Tibana, Ramires Alsamir ; Durigan, João Quaglioti ; Pereira, Guilherme Borges [UNESP] ; Franco, Octávio Luiz ; Royer, Carine ; Neves, Francisco de Assis Rocha ; Carvalho, Ana Carolina Andrade de ; Nóbrega, Otávio Toledo ; Haddad, Rodrigo ; Prestes, Jonato ; Marqueti, Rita de Cássia
Data: 2022
Identificador Persistente: http://hdl.handle.net/11449/222621
Origem: Oasisbr
Assunto(s): adipogenic; atrophy/hypertrophy signaling; exercise; gastrocnemius; intergenerational; proinflammatory cytokines; protein turnover
Descrição
Made available in DSpace on 2022-04-28T19:45:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-09-27
Although some studies have shown that a high-fat diet (HFD) adversely affects muscle extracellular matrix remodeling, the mechanisms involved in muscle trophism, inflammation, and adipogenesis have not been fully investigated. Thus, we investigated the effects of 8 weeks of paternal resistance training (RT) on gene and protein expression/activity of critical factors involved in muscle inflammation and remodeling of fathers and offspring (offspring exposed to standard chow or HFD). Animals were randomly distributed to constitute sedentary fathers (SF; n = 7; did not perform RT) or trained fathers (TF n = 7; performed RT), with offspring from mating with sedentary females. After birth, 28 male pups were divided into four groups (n = 7 per group): offspring from sedentary father submitted either to control diet (SFO-C) or high-fat diet (SFO-HF) and offspring from trained father submitted to control diet (TFO-C) or high-fat diet (TFO-HF). Our results show that an HFD downregulated collagen mRNA levels and upregulated inflammatory and atrophy pathways and adipogenic transcription factor mRNA levels in offspring gastrocnemius muscle. In contrast, paternal RT increased MMP-2 activity and decreased IL-6 levels in offspring exposed to a control diet. Paternal RT upregulated P70s6k and Ppara mRNA levels and downregulated Atrogin1 mRNA levels, while decreasing NFκ-B, IL-1β, and IL-8 protein levels in offspring exposed to an HFD. Paternal physical training influences key skeletal muscle remodeling pathways and inflammatory profiles relevant for muscle homeostasis maintenance in offspring submitted to different diets.
Laboratory of Molecular Analysis Faculty of Ceilândia Universidade de Brasília
Graduate Program in Rehabilitation Sciences Universidade de Brasília
Graduate Program of Sciences and Technology of Health Universidade de Brasília
Institute of Health Sciences – Universidade Paulista
Graduate Program in Health Sciences Faculdade de Medicine Universidade Federal do Mato Grosso (UFTM)
Interinstitutional Program of Post-Graduation in Physiological Sciences (UFSCar/UNESP) Department of Physiological Sciences Universidade Federal de São Carlos
Graduate Program in Genomics Science and Biotechnology Universidade Católica de Brasília
S-Inova Biotech Graduate Program in Biotechnology Universidade Católica Dom Bosco, Campo Grande
Laboratory of Molecular Pharmacology Faculty of Health Sciences Universidade de Brasília
Graduate Program of Medical Sciences Universidade de Brasília
Center for Tropical Medicine Universidade de Brasília
Graduate Program of Physical Education Universidade Católica de Brasilia
Interinstitutional Program of Post-Graduation in Physiological Sciences (UFSCar/UNESP) Department of Physiological Sciences Universidade Federal de São Carlos
