Autor(es): Rodrigues, Isabella C. P. ; Lopes, Éder S. N. ; Pereira, Karina D. [UNESP] ; Huber, Stephany C. ; Jardini, André Luiz ; Annichino-Bizzacchi, Joyce M. ; Luchessi, Augusto D. [UNESP] ; Gabriel, Laís P.
Data: 2022
Identificador Persistente: http://hdl.handle.net/11449/223724
Origem: Oasisbr
Made available in DSpace on 2022-04-28T19:52:45Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-12-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Vascular graft surgeries are often conducted in trauma cases, which has increased the demand for scaffolds with good biocompatibility profiles. Biodegradable scaffolds resembling the extracellular matrix (ECM) of blood vessels are promising vascular graft materials. In the present study, polyurethane (PU) was blended with ECM proteins collagen and elastin (Col-El) and gelatin (Gel) to produce fibrous scaffolds by using the rotary jet spinning (RJS) technique, and their effects on in vitro properties were evaluated. Morphological and structural characterization of the scaffolds was performed using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Micrometric fibers with nanometric rugosity were obtained. Col-El and Gel reduced the mechanical strength and increased the hydrophilicity and degradation rates of PU. No platelet adhesion or activation was observed. The addition of proteins to the PU blend increased the viability, adhesion, and proliferation of human umbilical vein endothelial cells (HUVECs). Therefore, PU-Col-El and PU-Gel scaffolds are promising biomaterials for vascular graft applications.
School of Applied Sciences University of Campinas, Rua Pedro Zaccaria, 1300, SP
School of Mechanical Engineering University of Campinas, Rua Mendeley, 200, SP
Institute of Biosciences São Paulo State University, SP
Hematology and Hemotherapy Center University of Campinas, SP
School of Chemical Engineering University of Campinas, SP
Institute of Biosciences São Paulo State University, SP
FAPESP: #2017/13273-6
