Author(s): Dionello, Nelson José Laurino ; Ferro, Jesus Aparecido ; Macari, Marcos [UNESP] ; Rutz, Fernando ; Furlan, Luiz Roberto [UNESP] ; Ferro, Maria Inês Tiraboschi
Date: 2022
Persistent ID: http://hdl.handle.net/11449/224454
Origin: Oasisbr
Subject(s): Acute heat stress; Hepatic and brain Hsp70; Northern Blotting and Western Blotting; Protein and mRNA Hsp70
Made available in DSpace on 2022-04-28T19:56:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2001-09-01
Eighty broiler chicks, Naked neck (Na/na) and Hubbard-Pettersen strains, were exposed to acute heat stress (36-37°C), from 2 to 5 days of age, and body weight and cloacal temperature were measured in the beginning and at the end of the heat stress period (5 h). Birds were sacrificed at the end and liver and brain samples were collected and analyzed using Western Blotting and Northern Blotting to quantify Hsp70 levels and mRNA Hsp70 transcript, respectively. The brain tissue had higher Hsp70 level than liver tissue. Before heat stress it was not observed difference in the Hsp70 expression between strains. After heat stress in the liver it was observed in increase in the Hsp70 level (naked neck birds at 4 and 5 days), and in the brain a reduced Hsp70 level was found when compared with the control birds (Naked neck birds at 2 days). The size of the mRNA Hsp70 transcript was 2.7 kb. The data of this experiment suggest that to the brain tissue heat stress affect Hsp70 at transcriptional and translational levels, and for hepatic tissue the effect is only at translational level, for different lines.
Prog. Pos Graduacao em Biotecnologia UFPel
Departamento de Tecnologia
Depto. de Morfol. e Fisiol. Animal Faculdade Cie. Agrarias Veterinarias Universidade Estadual Paulista-UNESP, Jaboticabal, SP. 14870-000
Departamento de Zootecnia Faculdade de Agronomia Eliseu Maciel Univ. Federal de Pelotas-UFPel, Pelotas, RS. 96100-000
Depto. de Melhoramento e Nutr. Anim. FMVZ-UNESP, Botucatu, SP
Depto. de Morfol. e Fisiol. Animal Faculdade Cie. Agrarias Veterinarias Universidade Estadual Paulista-UNESP, Jaboticabal, SP. 14870-000
Depto. de Melhoramento e Nutr. Anim. FMVZ-UNESP, Botucatu, SP
