Author(s): Regasini, Luis Octávio [UNESP] ; Cotinguiba, Fernando [UNESP] ; Morandim, Andreia De Araújo [UNESP] ; Kato, Massuo Jorge ; Scorzoni, Liliana [UNESP] ; Mendes-Giannini, Maria José [UNESP] ; Bolzani, Vanderlan Da Silva [UNESP] ; Furlan, Maysa [UNESP]
Date: 2022
Persistent ID: http://hdl.handle.net/11449/225537
Origin: Oasisbr
Subject(s): Antifungal; Antimicrobial; Candida; Cryptococcus neoformans; Piper arboreum; Piper tuberculatum; Piperaceae
Made available in DSpace on 2022-04-28T20:54:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-06-17
In the scope of our ongoing research on bioactive agents from natural sources, 24 extracts and fractions obtained from Piper arboreum Aub. and Piper tuberculatum Jacq. (Piperaceae) were screened for antifungal activity by using broth microdilution method. The current investigation reveals that P. arboreum extracts and fractions were more effective against Candida krusei and Candida parapsilosis than Cryptococcus neoformans. The growth of Candida albicans was weakly affected by all the tested extracts and fractions. The strongest effects were observed for hexane and ethyl acetate fractions from leaves of P. arboreum, with MIC values (in μ g/ml) of 15.6 and 31.2 μ g/ml against C. krusei, respectively. Additionally, phytochemical investigation of the hexane fraction of P. arboreum leaves furnished 3 pyrrolidine amides; piperyline, 4,5-dihydropiperyline and tetrahydropiperyline, which could be responsible, at least in part for the observed antifungal activity. The most active compound, tetrahydropiperyline, displayed MIC values of 15.6 μ g/ml against C. krusei, C. parapsilosis and C. neoformans. © 2009 Academic Journals.
Department of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP), Araraquara
Department of Fundamental Chemistry Institute of Chemistry São Paulo University (USP), São Paulo
Department of Clinical and Toxicological Analysis School of Pharmaceutical Sciences São Paulo State University (UNESP), Araraquara
Department of Organic Chemistry Institute of Chemistry São Paulo State University (UNESP), Araraquara
Department of Clinical and Toxicological Analysis School of Pharmaceutical Sciences São Paulo State University (UNESP), Araraquara
