Autor(es): Lima, D. S. ; Zárate-Bladés, C. R. ; Souza, P. R.M. ; Trombone, A. P. ; Santos, R. R. [UNESP] ; Brandão, I. T. ; Masson, A. P. ; Bonato, V. L. ; Coelho-Castelo, A. A.M. ; Sartori, A. [UNESP] ; Vendramini, M. ; Soares, E. G. ; Benvenutti, L. A. ; Silva, C. L. ; Coelho, Verônica
Data: 2022
Identificador Persistente: http://hdl.handle.net/11449/225607
Origem: Oasisbr
Assunto(s): Autoantibodies; Autoimmunity; DNA vaccine; Heat-shock protein
Made available in DSpace on 2022-04-28T20:55:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-01-01
Heat-shock proteins (HSPs) are currently one of the most promising targets for the development of immunotherapy against tumours and autoimmune disorders. This protein family has the capacity to activate or modulate the function of different immune system cells. They induce the activation of monocytes, macrophages and dendritic cells, and contribute to cross-priming, an important mechanism of presentation of exogenous antigen in the context of MHC class I molecules. These various immunological properties of HSP have encouraged their use in several clinical trials. Nevertheless, an important issue regarding these proteins is whether the high homology among HSPs across different species may trigger the breakdown of immune tolerance and induce autoimmune diseases. We have developed a DNA vaccine codifying the Mycobacterium leprae Hsp65 (DNAhsp65), which showed to be highly immunogenic and protective against experimental tuberculosis. Here, we address the question of whether DNAhsp65 immunization could induce pathological autoimmunity in mice. Our results show that DNAhsp65 vaccination induced antibodies that can recognize the human Hsp60 but did not induce harmful effects in 16 different organs analysed by histopathology up to 210 days after vaccination. We also showed that anti-DNA antibodies were not elicited after DNA vaccination. The results are important for the development of both HSP and DNA-based immunomodulatory agents. Copyright © by BIOLIFE, s.a.s.
Department of Biochemistry and Immunology Medicine School of Ribeirão Preto University of São Paulo, São Paulo
Department of Clinical Analyses Faculty of Pharmaceutical Sciences São Paulo State University, Araraquara, São Paulo
Department of Microbiology and Immunology Biosciences Institute São Paulo State University, Botucatu, São Paulo
Department of Pathology University of São Paulo Medical School, São Paulo
Department of Pathology School of Medicine of Ribeirão Preto University of São Paulo, Ribeirão Preto, São Paulo
Heart Institute (InCor) University of São Paulo Medical School, São Paulo
Institute for Investigation in Immunology National Institute of Science and Technology - INCT, São Paulo
Instituto do Coração Laboratório de Imunologia Universidade de São Paulo, Av Dr Eneas de Carvalho Aguiar, 44 bloco II, 9 andar, Cerqueira César, São Paulo - SP 05403-001
Department of Clinical Analyses Faculty of Pharmaceutical Sciences São Paulo State University, Araraquara, São Paulo
Department of Microbiology and Immunology Biosciences Institute São Paulo State University, Botucatu, São Paulo
