Author(s): Bouer, Andréa [UNESP] ; Werther, Karin [UNESP] ; Machado, Rosangela Zacarias [UNESP] ; Nakaghi, Andréa Cristina Higa [UNESP] ; Epiphanio, Sabrina ; Catão-Dias, José Luiz
Date: 2022
Persistent ID: http://hdl.handle.net/11449/225879
Origin: Oasisbr
Subject(s): ELISA; Indirect Fluorescence Assay; Primates; Toxoplasma gondii; Toxoplasmosis
Made available in DSpace on 2022-04-28T21:01:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-01-01
The Indirect Fluorescence Assay (IFA) and the indirect ELISA were comparatively used to detect IgG and IgM antibodies for Toxoplasma gondii in experimentally and naturally infected primates. In the experimentally infected group, antibodies of diagnostic value were detected at day 9 post-infection (PI) with the IFA (IgG and IgM) and with IgG-ELISA. IgM-ELISA detected antibodies for T. gondii starting at day 3 PI until the end of the experiment (102 days PI). Of the 209 naturally infected sera tested, from many zoos of State of Sao Paulo, 64.59 and 67.94% were positive in the IgG-IFA test and IgG-ELISA respectively. IgM-ELISA test detected seropositivity in 52.63% of the sera although IgM-IFA test detected it in only in 0.96% of the samples. The differential toxoplasmosis diagnosis was accomplished with Neospora caninum by IFA, observing 61 (29.2%) seropositive animals for this parasite and 149 (70.8%) negative. Sixty animals were positive for both T. gondii and N. caninum. Pneumonia, splenomegaly, and intestinal ulcers were macroscopically observed. Unremarkable interstitial pneumonia, enteritis, colitis, splenitis, and glomerulitis were microscopically observed. The immunohistochemical stain could not detect the presence of T. gondii in the tissues of the animals infected experimentally.
Faculdade de Medicina Veterinária Universidade de Cuiabá - UNIC
Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista - UNESP
Departamento de Patologia Faculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo - USP
Departamento de Patologia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista - UNESP
