Author(s): de Azevêdo Costa, Nivaldo ; Simão, Luis Carlos Valeriano ; dos Santos, Rogério Adriano ; Afonso, José Augusto Bastos ; Fagliari, José Jurandir [UNESP] ; da Cruz Cardoso, Elyzabeth ; Soares, Pierre Castro ; de Mendonça, Carla Lopes
Date: 2022
Persistent ID: http://hdl.handle.net/11449/226057
Origin: Oasisbr
Subject(s): Acute-phase proteins; Gram positive; Intramammary infection; Oligoelements; Ruminants; SDSPAGE
Made available in DSpace on 2022-04-28T21:25:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-05-01
The aim of the present study was to evaluate the effect of Staphylococcus aureus experimentally induced mastitis on proteinogram and serum concentrations of cupper, iron and zinc levels of Santa Ines primiparous ewes. The right mammary gland of ten healthy ewes was inoculated with 1,0×104 UFC/mL of S. aureus. Clinical examination and determination of serum concentrations of proteins by electrophoresis in polyacrylamide gel (SDS-PAGE), cupper, iron and zinc, as well plasma level of fibrinogen were measured before the inoculation (control) and 12h, 24h, 36h, 48h, 60h, 72h, 84h, 96h, 108h, 120h, 132h, 168h, 180h, 288h and 336h after bacteria inoculation. All animals experimentally infected presented clinical mastitis and subsequent loss of mammary gland function. The electrophoretogram allowed the identification of 23 proteins with molecular weights (MW) ranged from 26.000 to 185.000 daltons (Da) including acute-phase proteins, IgG and IgA. A significant increase (P<0,05) in haptoglobin, ceruloplasmin, IgG and IgA concentrations was observed. Antitrypsin and acid glicoprotein concentrations did not alter. The levels of iron and zinc decreased and the cupper concentration increased. A positive correlation between plasma fibrinogen and serum ceruloplasmin (r=0.74), haptoglobin (r=0.62) and IgA (r=0.62) was also identified. Results showed the importance of ceruloplasmin and haptoglobin as acute-phase proteins in ewes with intramammary infections and confirms fibrinogen as an inflammatory marker because its high correlation with specific proteins. The alterations in the serum levels of Cu, Fe and Zn suggest the action of inflammatory mediators triggered by S. aureus.
Programa de Pós-Graduação em Ciência Veterinária Universidade Federal Rural de Pernambuco (UFRPE), Av. Dom Manuel de Medeiros s/n, Dois Irmãos, Recife, PE 52171-030
Clínica de Bovinos Campus Garanhuns UFRPE, Av. Bom Pastor s/n, Boa Vista, Cx. Postal 152, Garanhuns, PE 55292-901
Departamento de Clínica e Cirurgia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp), Campus de Jaboticabal, Via de Acesso Prof., Jaboticabal, SP 14884-900
Faculdade de Medicina Veterinária Universidade Federal Fluminense (UFF), Rua Vital Brazil Filho 64, Santa Rosa, Niterói, RJ 24230-340
Departamento de Medicina Veterinária Universidade Federal Rural de Pernambuco(UFRPE), Recife, PE
Departamento de Clínica e Cirurgia Veterinária Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp), Campus de Jaboticabal, Via de Acesso Prof., Jaboticabal, SP 14884-900
