Author(s): Torres, A. F.C. ; Dantas, R. T. ; Menezes, R. R.P.P.B. ; Toyama, M. H. [UNESP] ; Filho, E. D. ; Oliveira, M. F. ; Nogueira, N. A.P. ; Oliveira, M. R. ; Monteiro, H. S.A. ; Martins, A. M.C.
Date: 2022
Persistent ID: http://hdl.handle.net/11449/226164
Origin: Oasisbr
Subject(s): Antimicrobial activity; Bothrops leucurus; L-amino oxidase
Made available in DSpace on 2022-04-28T21:39:41Z (GMT). No. of bitstreams: 0 Previous issue date: 2010-01-01
Some snake venom proteins present enzymatic activities, such as L-amino acid oxidase (LAAO). The aim of this paper was to investigate the effect of Bothrops leucurus total venom (BleuTV) and its fraction LAAO (BleuLAAO) on bacteria, yeast, and promastigote forms of Leishmania amazonensis and Leishmania chagasi, and epimastigote forms of Trypanosoma cruzi. BleuTV was isolated with a Protein Pack 5PW®(Waters Corporation, USA), and several fractions were obtained. BleuLAAO was purified to high molecular homogeneity, and its N-terminal amino acid sequence shared a high degree of amino acid conservation with other LAAOs. BleuTV inhibited Staphylococcus aureus growth in a dose-dependent manner, with a minimum inhibitory concentration (MIC) of 25 μg/mL, which corresponded to its minimum lethal concentration (MLC). BleuTV also inhibited the growth of promastigote forms of L. chagasi and L. amazonensis, with respective IC50 values of 1.94 μg/mL and 5.49 μg/mL. Furthermore, it repressed T. cruzi growth with an IC50 of 1.14 μg/mL. However, BleuLAAO did not inhibit the growth of the microorganisms studied and was not toxic to macrophages. BleuTV had low toxicity against macrophages at the concentrations studied. In conclusion, whole venom from Bothrops leucurus inhibited the growth of some microorganisms, including S. aureus, Leishmania sp., and T. cruzi. © CEVAP 2010.
Department of Clinical and Toxicological Analysis School of Pharmacy Federal University of Ceará, Fortaleza, Ceará State
Department of Physiology and Pharmacology Federal University of Ceará, Fortaleza, Ceará State
Laboratory of Cell Biology and Chemistry for Proteins and Peptides São Paulo Experimental Coast Campus São Paulo State University (UNESP - Univ Estadual Paulista), São Vicente, São Paulo State
Department of Biochemistry Institute of Biology State University of Campinas UNICAMP Campinas, São Paulo State
Department of Molecular Biology Center of Exact Sciences and Nature Federal University of Paraíba, João Pessoa, Paraíba State
Laboratory of Cell Biology and Chemistry for Proteins and Peptides São Paulo Experimental Coast Campus São Paulo State University (UNESP - Univ Estadual Paulista), São Vicente, São Paulo State
