Author(s): Regina, Magali ; Broetto, Fernando [UNESP] ; Giovannozzi-Sermanni, Giovanni ; Marabotini, Rosita ; Peranni, Cláudio ; Linde, Giani Andréa ; Colauto, Nelson Barros ; Paccola-Meirelles, Luiza Doretto
Date: 2022
Persistent ID: http://hdl.handle.net/11449/226386
Origin: Oasisbr
Subject(s): Laccase; Oxidative enzymes; Shiitake; Substrate; Waste
Made available in DSpace on 2022-04-28T22:37:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-01-01
Basidyomycete Lentinula edodes has its related enzymatic activity mainly on the agribusiness waste kind used as a substrate. The objective of this work was to verify the activity of oxidative enzymes lacase (Lac), lignina peroxidase (LiP) and manganese peroxidase (MnP) of three L. edodes strains, in stationary system, cultivated the 25 C°, in the absence of light, in substrates wtih 20% of bran of rice, 1% of CaCO3 and 79% of rice husk (CA), eucalyptus sawdust (SE), cassava bagasse (BM) and sugarcane bagasse (BC), adjusted to 60% of humidity. The Lac and MnP activities were bigger in eucalyptus sawdust (SE) and sugarcane bagasse (BC). The LiP activity was not induced for tested substrates. The rice husk (CA) and cassava bagasse (BM) substrates, although are not adequate to produce Lac or MnP, can be used as additives to increase the porosity, air availability and easy metabolism polysaccharides.
Centro de Ciências Agrárias Universidade Estadual de Londrina. (UEL)
Departamento de Química e Bioquímica Instituto de Biociências UNESP, Botucatu-SP
Dipartimento de Agrobiologia e Agrochimica Università Degli Studi della Tuscia, Viterbo-IT
Universidade Paranaense - UNIPAR Laboratório de Biologia Molecular, Umuarama-PR
Centro de Ciências Biológicas Universidade Estadual de Londrina (UEL), Londrina-PR
Departamento de Química e Bioquímica Instituto de Biociências UNESP, Botucatu-SP
