Autor(es): Sannomiya, Miriam [UNESP] ; Figueiredo, Maria E. [UNESP] ; Da Silva, Marcelo A. [UNESP] ; Rodrigues, Clenilson M. [UNESP] ; Dos Santos, Lourdes C. [UNESP] ; Souza-Brito, Alba R.M. ; Vilegas, Wagner [UNESP]
Data: 2022
Identificador Persistente: http://hdl.handle.net/11449/226479
Origem: Oasisbr
Assunto(s): Byrsonima basiloba; Counter-current chromatography; Flavonoids; Gradient elution
Made available in DSpace on 2022-04-29T00:13:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-01-01
A method for the isolation of three compounds from the infusion of leaves of Byrsonima basiloba A. Juss. by high-speed counter-current chromatography (HSCCC) was developed. This technique led to the separation of a novel compound, quercetin 3-O-α-L-rhamnopyranosyl-(1→3)-O-[α-L- rhamnopyranosyl-(1→6)]-β-D-allopyranoside, and two known compounds quercetin-3-O-α-L-rhamnopyranosyl-(1→6)-β-D-galactopyranoside and (+)-catechin in 4 h with purities of over 92%. The structures of the compounds were determined by one- and two-dimensional NMR spectroscopy and HPLC.
Departamento de Química Orgânica Instituto de Quimica UNESP, Rua Francisco Degni s/n, Bairro Quitandinha, CEP 14800-900 Araraquara, SP
Departamento de Fisiologia e Biofisica Instituto de Biologia, Unicamp-Campinas - SP
Departamento de Química Orgânica Instituto de Quimica UNESP, Rua Francisco Degni s/n, Bairro Quitandinha, CEP 14800-900 Araraquara, SP
