Autor(es):
Bastos, Natália Marins [UNESP] ; Rossi, Guilherme Fazan [UNESP] ; da Silva Leão, Beatriz Caetano [UNESP] ; Negrão, Fernanda ; Ferreira, Christina Ramires ; Vrisman, Dayane Priscila [UNESP] ; Rodrigues, Naiara Nantes [UNESP] ; Zorzetto, Mariana Furtado ; de Paula Freitas-Dell'Aqua, Camila [UNESP] ; Vantini, Roberta [UNESP] ; Monteiro, Fabio Morato [UNESP] ; Mercadante, Maria Eugênia Zerlotti ; Eberlin, Marcos Nogueira ; de Lima, Vera Fernanda Martins Hossepian [UNESP] ; Mingoti, Gisele Zoccal [UNESP]
Data: 2022
Identificador Persistente: http://hdl.handle.net/11449/229433
Origem: Oasisbr
Assunto(s): Bull; Lipid; Nongenetic inheritance; Nutrition; Paternal effect; Polyunsaturated fatty acids
Descrição
Made available in DSpace on 2022-04-29T08:32:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-11-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
The aim of this study was to examine the effects of long-term dietary supplementation of young Nellore bulls with rumen-protected polyunsaturated fatty acids (PUFAs) and of the inclusion of catalase in the semen extender on semen quality, in vitro sperm fertilizing ability, and intracytoplasmic lipid content in the resulting embryos. Twelve Nellore bulls were supplemented with rumen-protected PUFAs or with a basal diet from 14 to 24 months of age. The semen was collected at the end of supplementation. For cryopreservation, the ejaculate was divided into two equal volumes and catalase was added to the extender in one of the fractions. Thus, the experimental design consisted of a 2 × 2 factorial scheme with two diets (control and PUFA) and two extenders (Cat+ and Cat-). Total motility and the percentage of rapid cells in fresh semen were negatively affected by dietary supplementation with PUFAs (P < 0.05), but these effects did not persist after freezing. The frozen/thawed semen of animals fed PUFAs exhibited an increase in the percentages of damaged plasma and acrosomal membranes, as well as an increase in the proportion of lipids ions at m/z 578 and m/z 757 detected by MALDI-MS. Nevertheless, there was no effect of the treatments on in vitro embryo development. However, embryos derived from bulls supplemented with PUFAs exhibited higher lipid accumulation compared to control (P < 0.05). In conclusion, PUFA supplementation promoted worsening of semen quality without affecting the in vitro sperm fertilizing ability; however, the paternal diet affected the intracytoplasmic lipid content in the resulting embryos.
São Paulo State University (UNESP) Department of Animal Reproduction Graduate Program in Genetics and Animal Breeding School of Agrarian and Veterinary Sciences Campus Jaboticabal
São Paulo State University (UNESP) Department of Animal Reproduction Graduate Program in Veterinary Medicine School of Agrarian and Veterinary Sciences Campus Jaboticabal
Proteomics Center of Excellence Northwestern University, 2170 Campus Dr
ThoMSon Mass Spectrometry Laboratory Institute of Chemistry University of Campinas Cidade Universitaária Zeferino Vaz, Campinas
Department of Chemistry Purdue University
Instituto de Zootecnia (IZ/APTA), Sertãozinho
São Paulo State University (UNESP) School of Veterinary Medicine and Animal Science, Campus Botucatu
São Paulo State University (UNESP) School of Veterinary Medicine Laboratory of Reproductive Physiology, Campus Araçatuba
São Paulo State University (UNESP) Department of Animal Reproduction Graduate Program in Genetics and Animal Breeding School of Agrarian and Veterinary Sciences Campus Jaboticabal
São Paulo State University (UNESP) Department of Animal Reproduction Graduate Program in Veterinary Medicine School of Agrarian and Veterinary Sciences Campus Jaboticabal
São Paulo State University (UNESP) School of Veterinary Medicine and Animal Science, Campus Botucatu
São Paulo State University (UNESP) School of Veterinary Medicine Laboratory of Reproductive Physiology, Campus Araçatuba
FAPESP: #2013/13696–3
FAPESP: #2015/06733–5
FAPESP: #2019/11174–6
CNPq: #307416/2015–1
CNPq: #314136/2018–5
CAPES: 001
