Author(s): Herkenhoff, Marcos Edgar [UNESP] ; Bovolenta, Luiz A. [UNESP] ; Broedel, Oliver ; dos Santos, Lucilene D. [UNESP] ; de Oliveira, Arthur C. [UNESP] ; Chuffa, Luiz G.A. [UNESP] ; Ribeiro, Amanda de O. [UNESP] ; Lupi, Luiz A. [UNESP] ; Dias, Marco A.D. ; Hilsdorf, Alexandre W.S. ; Frohme, Marcus ; Pinhal, Danillo [UNESP]
Date: 2022
Persistent ID: http://hdl.handle.net/11449/229918
Origin: Oasisbr
Subject(s): ESI-Q-TOF; Growth; miRNA; Nile tilapia; Proteome; RNA-Seq
Made available in DSpace on 2022-04-29T08:36:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-11-01
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Nile tilapia (Oreochromis niloticus) is a species of worldwide importance for aquaculture. A crossbred lineage was developed through introgressive backcross breeding techniques and combines the high growth performance of the Chitralada (CHIT) lwith attractive reddish color of the Red Stirling (REDS) strains. Since the crossbreed has an unknown genetically improved background, the objective of this work was to characterize expression signatures that portray the advantageous phenotype of the crossbreeds. We characterized the microRNA transcriptome by high throughput sequencing (RNA-seq) and the proteome through mass spectrometry (ESI-Q-TOF-MS) and applied bioinformatics for the comparative analysis of such molecular data on the three strains. Crossbreed expressed a distinct set of miRNAs and proteins compared to the parents. They comprised several microRNAs regulate traits of economic interest. Proteomic profiles revealed differences between parental and crossbreed in expression of proteins associated with glycolisis. Distinctive miRNA and protein signatures contribute to the phenotype of crossbreed.
Department of Chemical and Biological Sciences Institute of Biosciences of Botucatu São Paulo State University (UNESP)
Department of Biophysics and Pharmacology Institute of Biosciences of Botucatu São Paulo State University (UNESP)
Division Molecular Biotechnology and Functional Genomics Technical University of Applied Sciences Wildau
Center for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)
Department of Structural and Functional Biology Institute of Biosciences of Botucatu São Paulo State University (UNESP)
Unit of Biotechnology University of Mogi das Cruzes
Department of Animal Sciences Federal University of Lavras
Department of Chemical and Biological Sciences Institute of Biosciences of Botucatu São Paulo State University (UNESP)
Department of Biophysics and Pharmacology Institute of Biosciences of Botucatu São Paulo State University (UNESP)
Center for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)
Department of Structural and Functional Biology Institute of Biosciences of Botucatu São Paulo State University (UNESP)
