Author(s):
Moro Lima Dos Santos, Ana Cláudia [UNESP] ; Monteiro, Aydir Cecília Marinho [UNESP] ; Barbosa, Thaís Alves [UNESP] ; Riboli, Danilo Flávio Moraes [UNESP] ; Camargo, Carlos Henrique ; Ferreira, Adriano Martison [UNESP] ; Mondelli, Alessandro Lia [UNESP] ; Montelli, Augusto Cezar [UNESP] ; Hernandes, Rodrigo Tavanelli [UNESP] ; Ribeiro de Souza da Cunha, Maria de Lourdes [UNESP] ; Barretti, Pasqual [UNESP]
Date: 2022
Persistent ID: http://hdl.handle.net/11449/230370
Origin: Oasisbr
Subject(s): Acinetobacter; Antimicrobial resistance; Biofilm; Clonal profile; Nonfermenting Gram-negative bacilli; Peritoneal dialysis; Peritonitis; Pseudomonas; Virulence factors
Description
Made available in DSpace on 2022-04-29T08:39:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-02-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
(1) Background: Peritonitis due to nonfermenting Gram-negative bacilli (NF-GNB) is a dramatic complication of peritoneal dialysis (PD) with bad outcomes. Previous studies of PD-related peritonitis due to Pseudomonas species have shown a low-resolution rate, without a high resistance rate to antipseudomonal antibiotics. This suggests that bacterial virulence factors can act and influence peritonitis evolution. This study aimed to describe the microbiological characteristics of NF-GNB causing PD-related peritonitis and analyze their influence on the outcome. (2) Methods: We analyze the 48 isolates from NF-GNB peritonitis, which were stored in our culture collection regarding bacterial resistance, biofilm, and other virulence factors’ production, and clonal profile. Additionally, we collected data on treatment and outcomes from patients’ clinical registers. (3) Results: The etiologies were species of Pseudomonas (50%), Acinetobacter (36%), and other NF-GNB (14%). There was a high (75%) proportion of biofilm producer lineages. The in vitro susceptibility rate of Pseudomonas spp. to amikacin, ciprofloxacin, and ceftazidime was significantly greater than that of Acinetobacter spp. and other species; however, there was a similar low-resolution rate (<45%) among the episodes attributable to them. Pseudomonas species have a polyclonal profile, while we found a clone of five multiresistant Acinetobacter baumannii over an 8-year interval (2000–2008), which suggest an origin from the healthcare environment. (4) Conclusions: We are not able to identify any predictor of outcome, but it is possible that biofilm and others virulence factors can act in concert and contribute to the bad outcome.
Departamento de Ciências Químicas e Biológicas Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), SP
Centro de Bacteriologia Instituto Adolfo Lutz, SP
Hospital das Clínicas da Faculdade de Medicina de Botucatu, SP
Departamento de Clínica Médica Faculdade de Medicina Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), SP
Departamento de Ciências Químicas e Biológicas Instituto de Biociências Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), SP
Hospital das Clínicas da Faculdade de Medicina de Botucatu, SP
Departamento de Clínica Médica Faculdade de Medicina Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP), SP
FAPESP: 2013/21379-8
