Author(s): Corsino, Joaquim [UNESP] ; Alécio, Alberto Camilo [UNESP] ; Ribeiro, Maria Lücia [UNESP] ; Furlan, Maysa [UNESP] ; Pereira, Ana Maria S. ; Duarte, Ines Batista ; França, Suzelei Castro
Date: 2022
Persistent ID: http://hdl.handle.net/11449/231683
Origin: Oasisbr
Subject(s): 22β-hydroxymaitenin; Callus; Cell culture; Maitenin; Maytenus aquifolium; Quantitative reversed-phase high-performance liquid chromatography
Made available in DSpace on 2022-04-29T08:46:54Z (GMT). No. of bitstreams: 0 Previous issue date: 1998-01-01
Explants of Maytenus aquifolium were induced to form callus and, subsequently, suspension cultures. The isolation of natural products from callus led to the identification of the cytotoxic triterpene quinonemethides, maitenin (1) and 22β-hydroxymaitenin (2). A rapid, sensitive and reliable reversed-phase high-performance liquid chromatography method was developed using a C18 column and isocratic elution for the determination of 1 and 2. The elaborated method gave well-resolved peaks for these compounds with good detection response and linearity in the range of 0.08-72.0 μg. The quantification of 1 and 2 was performed by an external standard method.
Instituto de Química Universidade Estadual Paulista, CP. 355, 14800-900, Araraquara-SP
Departamento de Biotecnologia UNAERP, 14096-380, Ribeirão Preto-SP
Departamento de Morfologia CCBS UFMS, CP. 649, 79070-900, Campo Grande-MS
Instituto de Química Universidade Estadual Paulista, CP. 355, 14800-900, Araraquara-SP
