Autor(es): Gallego, C. ; Pardo, Margarita [UNESP] ; Camargos, Aline ; Sousa, D. [UNESP] ; Lenz, María I. ; Ramos, A. A. [UNESP] ; Jorge, A. M. ; Da Cruz Ladim, Fernanda [UNESP] ; Oba, Eunice [UNESP]
Data: 2022
Identificador Persistente: http://hdl.handle.net/11449/232166
Origem: Oasisbr
Assunto(s): Biochemical profile; Follicular fluid; Post-partum
Made available in DSpace on 2022-04-29T08:49:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-12-01
Twenty six Murrah female river buffaloes, between 45 and 70 d post-partum, empty, multiparae, with an average live weight of 675 ± 56 kg, and average body condition of 3.5 points, in a 1 to 5 scale, were used to determine the concentrations of glucose, cholesterol, total protein and insulin-like growth factor type I(IGF-I) in the follicular fluid. The fluid was collected from dominant follicles, with diameters between 8 and 12 mm, by in vivo follicular aspiration. The oestrous cycle stage was not taken into account. The wave of follicular development was synchronized six days prior to the collection. Biochemical analyses of glucose and cholesterol were performed by the enzymatic colorimetric method with the utilization of commercial kits of Glicose (GOD-PAP) and Cholesterol (CHOD-PAP) (Kovalent), respectively. For the determination of total protein, the commercial kit total Protein (Kovalent), method Biuret, was employed. Readings were carried out through absorption spectrophotometry with visible light. Through the radioimmunoanalysis (RIA) technique the concentration of IGF-I was obtained using commercial kits of IRMA IGF-I (IMMUNOTECH). Descriptive statistics was used, by applying the PROC MEANS procedure of the SAS (2009) statistical package. Glucose concentrations (4.0 ± 0.75 mmol/L) and IGF-I (340 ± 129.83 ng/mL) showed higher values in female river buffaloes and dairy cows regarding those reported in other studies. However, cholesterol levels (0.51 ± 0.12 mmol/L) and total proteins (58.4 ± 4.43 g/L) were lower. Results indicate that there is a relationship between the concentration of biochemical indicators, the nutritional aspects, the diameter of the aspired follicles and the productive period.
Instituto de Ciencia Animal, Apartado Postal 24, San-José de las Lajas, Mayabeque
Campus de Botucatú Universidad Estadual Paulista Julio, Mesquita Filho
Campo Grande Universidad Federal de Mato Grosso, Mato Grosso
Campus de Botucatú Universidad Estadual Paulista Julio, Mesquita Filho
