Author(s): Dilarri, Guilherme [UNESP] ; Zamuner, Caio Felipe Cavicchia [UNESP] ; Bacci, Mauricio [UNESP] ; Ferreira, Henrique [UNESP]
Date: 2022
Persistent ID: http://hdl.handle.net/11449/233200
Origin: Oasisbr
Subject(s): Citrus canker; Orange; Packinghouse; Post-harvest sanitization; Sodium hypochlorite; Xanthomonas citri subsp. citri
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Xanthomonas citri (X. citri) is a quarentenary plant pathogen and the causal agent of the citrus canker. X. citri forms biofilms and remains fixed on the surface of plant tissues, especially on leaves and fruits. Considering this, all the citrus fruits have to be sanitized before they can be commercialized. NaOCl is the main sanitizer used to decontaminate fruits in the world. Due to its toxicity, treatment with NaOCl is no longer accepted by some Europe Union countries. Therefore, the aim of this work was to evaluate potassium bicarbonate (KHCO3), calcium hydroxide (Ca(OH)2), calcium hypochlorite (Ca(OCl)2) and peracetic acid (CH3CO3H) as alternatives to NaOCl for the sanitization of citrus fruit. By monitoring cell respiration and bacterial growth, we determined that peracetic acid and calcium hypochlorite exhibit bactericidal action against X. citri. Time-response growth curves and membrane integrity analyses showed that peracetic acid and calcium hypochlorite target the bacterial cytoplasmatic membrane, which is probably responsible for cell death in the first minutes of contact. The simulation of the sanitization process of citrus fruit in packinghouses showed that only peracetic acid exhibited a performance comparable to NaOCl. Among the tested compounds, peracetic acid constitutes an efficient and safer alternative to NaOCl.
Department of General and Applied Biology São Paulo State University (UNESP), Av. 24A, 1515, Bela Vista
Department of General and Applied Biology São Paulo State University (UNESP), Av. 24A, 1515, Bela Vista
