Autor(es): Dos Santos, Carla Adriana ; Hernandes, Rodrigo Tavanelli [UNESP] ; Cunha, Marcos Paulo Vieira ; Nagamori, Filipe Onishi ; Gonçalves, Claudia Regina ; Sacchi, Claudio Tavares ; Tiba-Casas, Monique Ribeiro ; Camargo, Carlos Henrique
Data: 2022
Identificador Persistente: http://hdl.handle.net/11449/233382
Origem: Oasisbr
Assunto(s): Antimicrobial resistance; Escherichia coli; Polymyxin B; Uropathogenic Escherichia coli; Whole-genome sequencing
Made available in DSpace on 2022-05-01T08:15:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-08-01
Background: Uropathogenic Escherichia coli (UPEC) are frequent pathogens worldwide, impacting on the morbidity and economic costs associated with antimicrobial treatment. Objectives: We report two novel mutations associated with polymyxin-B resistance in an UPEC isolate collected in 2019. Methods: Isolate was submitted to antimicrobial susceptibility testing including broth microdilution for polymyxin B. Whole genome was sequenced and analyzed. Results: Polymyxin-B total inhibition occurred at 16 mg/L (resistant). UPEC isolate was assigned to the phylogroup D, serotype O117:H4, and Sequence Type 69. mcr genes were not detected, but two novel mutations in the pmrA/basS (A80S) and pmrB/basR (D149N) genes were identified. Conclusions: The occurrence of non-mcr polymyxin resistance in E. coli from extraintestinal infections underscores the need of a continuous surveillance of this evolving pathogen.
Instituto Adolfo Lutz
Instituto de Biociências de Botucatu Universidade Estadual Paulista júlio de Mesquita Filho
Faculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo
Instituto de Biociências de Botucatu Universidade Estadual Paulista júlio de Mesquita Filho
