Autor(es): Picchi, Simone Cristina ; de Souza e Silva, Mariana ; Saldanha, Luiz Leonardo [UNESP] ; Ferreira, Henrique [UNESP] ; Takita, Marco Aurélio ; Caldana, Camila ; de Souza, Alessandra Alves
Data: 2022
Identificador Persistente: http://hdl.handle.net/11449/233404
Origem: Oasisbr
Made available in DSpace on 2022-05-01T08:15:18Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-12-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
N-Acetylcysteine (NAC) is an antioxidant, anti-adhesive, and antimicrobial compound. Even though there is much information regarding the role of NAC as an antioxidant and anti-adhesive agent, little is known about its antimicrobial activity. In order to assess its mode of action in bacterial cells, we investigated the metabolic responses triggered by NAC at neutral pH. As a model organism, we chose the Gram-negative plant pathogen Xanthomonas citri subsp. citri (X. citri), the causal agent of citrus canker disease, due to the potential use of NAC as a sustainable molecule against phytopathogens dissemination in citrus cultivated areas. In presence of NAC, cell proliferation was affected after 4 h, but damages to the cell membrane were observed only after 24 h. Targeted metabolite profiling analysis using GC–MS/TOF unravelled that NAC seems to be metabolized by the cells affecting cysteine metabolism. Intriguingly, glutamine, a marker for nitrogen status, was not detected among the cells treated with NAC. The absence of glutamine was followed by a decrease in the levels of the majority of the proteinogenic amino acids, suggesting that the reduced availability of amino acids affect protein synthesis and consequently cell proliferation.
Centro de Citricultura “Sylvio Moreira” – Instituto Agronômico de Campinas Cordeirópolis
Departamento de Bioquímica e Microbiologia Instituto de Biociências Universidade Estadual Paulista
Laboratório Nacional de Ciência e Tecnologia do Bioetanol - Centro Nacional de Pesquisa em Energia e Materiais, Campinas
Max-Planck-Institut Für Molekulare Pflanzenphysiologie Wissenschaftspark Golm, Am Mühlenberg 1
Departamento de Bioquímica e Microbiologia Instituto de Biociências Universidade Estadual Paulista
FAPESP: 2013/01395-9
FAPESP: 2013/10957-0
FAPESP: 2014/50880-0
FAPESP: 2018/10734-5
CNPq: 465440/2014-2
