Author(s):
Stricker, Priscila Elias Ferreira ; de Souza Dobuchak, Daiany ; Irioda, Ana Carolina ; Mogharbel, Bassam Felipe ; Franco, Celia Regina Cavichiolo ; de Souza Almeida Leite, José Roberto ; de Araújo, Alyne Rodrigues ; Borges, Felipe Azevedo [UNESP] ; Herculano, Rondinelli Donizetti [UNESP] ; de Oliveira Graeff, Carlos Frederico [UNESP] ; Chachques, Juan Carlos ; de Carvalho, Katherine Athayde Teixeira
Date: 2022
Persistent ID: http://hdl.handle.net/11449/233410
Origin: Oasisbr
Subject(s): Biopolymer; Blood; Cholinergic neurons; Membrane; Mesenchymal stem cells; Umbilical cord
Description
Made available in DSpace on 2022-05-01T08:44:34Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-08-01
This study aimed to differentiate human mesenchymal stem cells (hMSCs) from the human umbilical cord in cholinergic-like neurons using a natural membrane. The isolation of hMSCs from Wharton’s jelly (WJ) was carried out using “explant” and mononuclear cells by the density gradient from umbilical blood and characterized by flow cytometry. hMSCs were seeded in a natural functional biopolymer membrane to produce neurospheres. RT-PCR was performed on hMSCs and neurospheres derived from the umbilical cord. Neural precursor cells were subjected to a standard cholinergic-like neuron differentiation protocol. Dissociated neurospheres, neural precursor cells, and cholinergic-like neurons were characterized by immunocytochemistry. hMSCs were CD73+, CD90+, CD105+, CD34-and CD45-and demonstrated the trilineage differentiation. Neurospheres and their isolated cells were nestin-positive and expressed NESTIN, MAP2, ßIII-TUBULIN, GFAP genes. Neural precursor cells that were differentiated in cholinergic-like neurons expressed ßIII-TUBULIN protein and choline acetyltransferase enzyme. hMSCs seeded on the natural membrane can differentiate into neurospheres, obtaining neural precursor cells without growth factors or gene transfection before cholinergic phenotype differentiation.
Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department Child and Adolescent Health Research and Pequeno Príncipe Faculties Pelé Pequeno Príncipe Institute
Cell Biology Department Federal University of Paraná
Research Center in Applied Morphology and Immunology NuPMIA Faculty of Medicine University of Brasília
Biodiversity and Biotechnology Research Parnaíba Delta Federal University
Faculty of Pharmaceutics Sciences São Paulo State University (UNESP)
Physics Department São Paulo State University (UNESP)
Laboratory Biosurgical Research Cardiovascular Division Pompidou Hospital University of Paris
Faculty of Pharmaceutics Sciences São Paulo State University (UNESP)
Physics Department São Paulo State University (UNESP)
