Author(s):
Maiolino, Sérgio Ricardo [UNESP] ; Cortez, Adriana ; Langoni, Helio [UNESP] ; Giuffrida, Rogério ; dos Santos, Joeleni Rosa [UNESP] ; de Nardi Júnior, Geraldo ; Lara, Gustavo Henrique Batista [UNESP] ; Motta, Rodrigo Garcia [UNESP] ; Chacur, Marcelo George Mungai ; Monteiro, Fábio Morato ; Heinemann, Marcos Bryan ; de Souza Filho, Antônio Francisco ; de Souza Araújo Martins, Lorrayne [UNESP] ; Bello, Thaís Spessotto [UNESP] ; Ribeiro, Márcio Garcia [UNESP]
Date: 2022
Persistent ID: http://hdl.handle.net/11449/233448
Origin: Oasisbr
Subject(s): Bull leptospirosis; Molecular diagnosis; Reproduction by natural means; Seroprevalence
Description
Made available in DSpace on 2022-05-01T08:44:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01
The present study investigated the serum microscopic agglutination test (MAT) among 203 bovine bulls with reproduction by natural means, without apparent signs of orchitis or inflammation of accessory reproductive glands. Simultaneously, the semen of all bulls was subjected to sperm viability analysis and PCR based on the 16S rRNA gene. PCR-positive results of semen samples were confirmed by sequencing. A modified seminal plasma agglutination (MSPA) test, replacing the blood serum of all bulls in the MAT with seminal plasma was performed as well. Eight (8/203 = 3.9%) semen samples from bulls were considered nonviable (necrospermia and azoospermia) without relation to the PCR diagnosis. No agglutinin titers were identified in MSPA test. A high frequency (132/203 = 65%) of leptospiral agglutinin titers was identified in the MAT, particularly for the Sejroe serogroup (Hardjo CTG, 100/203 = 49.3%; Wolffi 74/203 = 36.4%; Guaricura 72/203 = 35.5%; and Hardjoprajitno 56/203 = 27.6%). Three (3/203 = 1.5%) semen samples of bulls were positive in the PCR, but these results were not confirmed by sequencing. The high frequency of serovars from the Sejroe serogroup typically adapted to bovines indicates the need for measures for the prophylaxis/control of the pathogen on the sampled farms. Discrepancies among the MAT, sperm viability, and molecular detection of leptospires in semen highlight the need for a combination of methods to diagnose leptospirosis in bovine bulls. To our knowledge, modified seminal plasma agglutination is described for the first time here to investigate anti-Leptospira antibodies produced locally in the genital tract in the diagnosis of bovine leptospirosis among bulls that reproduce by natural means.
Department of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine an Animal Science São Paulo State University - UNESP
Santo Amaro University - UNISA
Faculty of Veterinary Medicine University of Oeste Paulista
Technology Faculty - FATEC
APTA Beef Cattle Center Institute of Animal Science
University of São Paulo - USP
Department of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine an Animal Science São Paulo State University - UNESP
