Author(s): Sevá, Anaiá da Paixão ; Brandão, Ana Pérola Drulla ; Godoy, Silvia Neri ; Soares, Rodrigo Martins ; Langoni, Helio [UNESP] ; Rodrigues, Bruna Cristine ; Gava, Mariana Zanchetta E [UNESP] ; Zanotto, Paula Ferraz de Camargo [UNESP] ; Jimenez-Villegas, Tatiana ; Hiramoto, Roberto ; Ferreira, Fernando
Date: 2022
Persistent ID: http://hdl.handle.net/11449/233600
Origin: Oasisbr
Made available in DSpace on 2022-05-01T09:30:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01
INTRODUCTION: Visceral leishmaniasis (VL) is an important zoonosis in Brazil. Previous identification of parasitized dogs can also help prevent the disease in humans, even in non-endemic areas of the country. The Brazilian Ministry of Health recommends diagnosis in dogs using a DPP® (rapid test) as a screening test and an immunoenzymatic assay (ELISA) as a confirmatory test (DPP®+ELISA), and culling infected dogs as a legal control measure. However, the accuracy of these serological tests has been questioned. METHODS: VL in dogs was investigated in a non-endemic area of the São Paulo state for three consecutive years, and the performances of different diagnostic tests were compared. RESULTS: A total of 331 dog samples were collected in 2015, 373 in 2016, and 347 in 2017. The seroprevalence by DPP®+ELISA was 3.3, 3.2, and 0.3%, respectively, and by indirect immunofluorescence assay (IFA), it was 3.0, 5.6, and 5.5%, respectively. ELISA confirmed 18.4% of DPP® positive samples. The concordance between the IFA and DPP® was 83.9%. The concordance between IFA and DPP®+ELISA was 92.9%. A molecular diagnostic test (PCR) was performed in 63.2% of the seropositive samples, all of which were negative. CONCLUSIONS: In non-endemic areas, diagnostic tests in dogs should be carefully evaluated to avoid false results.
Universidade de São Paulo Departamento de Veterinária Preventiva e Saúde Animal
Universidade Estadual de Santa Cruz, Departamento de Ciências Agrárias e Ambientais, Ilhéus, BA, Brasil
Instituto Chico Mendez de Conservação da Biodiversidade
Universidade Estadual Paulista Júlio de Mesquita Filho Departamento de Higiene Veterinária e Saúde Pública
Instituto Adolfo Lutz
Universidade Estadual Paulista Júlio de Mesquita Filho Departamento de Higiene Veterinária e Saúde Pública
