Author(s): Mendes, Carolina Rosai [UNESP] ; Dilarri, Guilherme [UNESP] ; Forsan, Carolina Froes [UNESP] ; Sapata, Vinícius de Moraes Ruy [UNESP] ; Lopes, Paulo Renato Matos [UNESP] ; de Moraes, Peterson Bueno ; Montagnolli, Renato Nallin [UNESP] ; Ferreira, Henrique [UNESP] ; Bidoia, Ederio Dino [UNESP]
Date: 2022
Persistent ID: http://hdl.handle.net/11449/234152
Origin: Oasisbr
Made available in DSpace on 2022-05-01T13:41:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-12-01
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Zinc oxide nanoparticles (ZnO NPs) are one of the most widely used nanoparticulate materials due to their antimicrobial properties, but their main mechanism of action (MOA) has not been fully elucidated. This study characterized ZnO NPs by using X-ray diffraction, FT-IR spectroscopy and scanning electron microscopy. Antimicrobial activity of ZnO NPs against the clinically relevant bacteria Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, and the Gram-positive model Bacillus subtilis was evaluated by performing resazurin microtiter assay (REMA) after exposure to the ZnO NPs at concentrations ranging from 0.2 to 1.4 mM. Sensitivity was observed at 0.6 mM for the Gram-negative and 1.0 mM for the Gram-positive cells. Fluorescence microscopy was used to examine the interference of ZnO NPs on the membrane and the cell division apparatus of B. subtilis (amy
Department of General and Applied Biology Sao Paulo State University (UNESP), 24-A Avenue, 1515, SP
College of Technology and Agricultural Sciences Sao Paulo State University (UNESP), SP‑294, km 651, SP
School of Technology State University of Campinas (UNICAMP), 6122, SP
Department of Natural Sciences Mathematics and Education Agricultural Sciences Centre Federal University of Sao Carlos (UFSCar), SP-330, km 174, SP
Department of General and Applied Biology Sao Paulo State University (UNESP), 24-A Avenue, 1515, SP
College of Technology and Agricultural Sciences Sao Paulo State University (UNESP), SP‑294, km 651, SP
FAPESP: 2015/50162-2
FAPESP: 2017/07306-9
