Autor(es): Tetzner, Tatiane Almeida Drummond [UNESP] ; Saraiva, Naiara Zoccal [UNESP] ; Perecin, Felipe ; Niciura, Simone Cristina Méo ; Ferreira, Christina Ramires ; Oliveira, Clara Slade [UNESP] ; Garcia, Joaquim Mansano [UNESP]
Data: 2014
Identificador Persistente: http://hdl.handle.net/11449/2599
Origem: Oasisbr
Assunto(s): bovine embryo; in vitro culture; Ovalbumin; Protein source
Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T19:07:48Z No. of bitstreams: 1 S1516-35982011001000010.pdf: 149616 bytes, checksum: 07d1ccdbc572102f20e627010a3cb293 (MD5)
Made available in DSpace on 2013-08-22T19:07:48Z (GMT). No. of bitstreams: 1 S1516-35982011001000010.pdf: 149616 bytes, checksum: 07d1ccdbc572102f20e627010a3cb293 (MD5) Previous issue date: 2011-10-01
Made available in DSpace on 2013-09-30T17:55:15Z (GMT). No. of bitstreams: 2 S1516-35982011001000010.pdf: 149616 bytes, checksum: 07d1ccdbc572102f20e627010a3cb293 (MD5) S1516-35982011001000010.pdf.txt: 36226 bytes, checksum: 7ffd1ab0b689346846db54ca0121e0c3 (MD5) Previous issue date: 2011-10-01
Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:15:28Z No. of bitstreams: 2 S1516-35982011001000010.pdf: 149616 bytes, checksum: 07d1ccdbc572102f20e627010a3cb293 (MD5) S1516-35982011001000010.pdf.txt: 36226 bytes, checksum: 7ffd1ab0b689346846db54ca0121e0c3 (MD5)
Made available in DSpace on 2014-05-20T13:15:28Z (GMT). No. of bitstreams: 2 S1516-35982011001000010.pdf: 149616 bytes, checksum: 07d1ccdbc572102f20e627010a3cb293 (MD5) S1516-35982011001000010.pdf.txt: 36226 bytes, checksum: 7ffd1ab0b689346846db54ca0121e0c3 (MD5) Previous issue date: 2011-10-01
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Embryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the supplement used for IVM. Supplementation with OVA for IVM+IVC (20.40%) and for IVF (22.15%) was inferior to supplementation with FCS for IVM+IVC (30.47%) and with BSA for IVF (28.91%) for blastocyst development. Hatching rates were lower using OVA for IVM+IVC (23.02%) and for IVF (28.93%) compared with FCS and BSA under the same conditions (40.78 and 34.82%, respectively) and BSA for IVF (36.82%). Supplementation with OVA for IVM+IVC and IVF resulted in reduced inner cell mass, trophectoderm cells and total blastocyst cell numbers (17.29, 37.88, and 55.17, respectively). In conclusion, OVA is a protein source for bovine in vitro embryo production, although the quantity and quality of bovine blastocysts using only ovalbumin in the entire in vitro production process are lower than those obtained in the presence of FCS and BSA, when used as supplements in any step of bovine in vitro embryo production.
UNESP FCAV Departamento de Reprodução Animal
USP Faculdade de Zootecnia e Engenharia de Alimentos
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Pecuária Sudeste
UNICAMP Instituto de Química Departamento de Química Orgânica
UNESP FCAV Departamento de Reprodução Animal
