Author(s): da Silva, Marcelo Aparecido [UNESP] ; Lima Cardoso, Claudia Andrea ; Vilegas, Wagner [UNESP] ; Santos, Lourdes Campaner dos [UNESP]
Date: 2014
Persistent ID: http://hdl.handle.net/11449/26159
Origin: Oasisbr
Subject(s): Eriocaulaceae; liquid chromatography; quantification; flavonoids
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Quantification of prepared samples by analysis using high performance liquid chromatography with DAD detection was developed to analyze rutin, 6-methoxyapigenin, and 6-methoxyapigenin-7-O-beta-D-glucopyranoside isolated from methanolic extracts of different genus: Syngonanthus, Leiothix and Eriocaulon (Eriocaulaceae). The linearity, accuracy, and the inter-day precision of the procedure were evaluated. The calibration curves were linear. The recoveries of the flavonoids in the samples analyzed were 96.3% to 98.5%. The percentage coefficient of variation for the quantitative analysis of the flavonoids in the analyses of the samples was under 5%. The antimicrobial activity of the five methanol extracts of these Eriocaulaceae species was assayed against the microorganisms Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Salmonella setubal, Saccharomyces cerevisiae and Candida albicans. Measured MIC values ranged from 1.25 to 10.00 mg/mL. The flavonoid contents suggest that Eriocaulaceae species may be a promising source of compounds to produce natural phytomedicines.
UNESP Univ Estadual Paulista, Inst Quim, Dept Quim Organ, BR-14800900 São Paulo, Brazil
Mato Grosso Sul State Univ, Course Chem, UEMS, BR-79804970 Dourados, Mato Grosso Su, Brazil
UNESP Univ Estadual Paulista, Inst Quim, Dept Quim Organ, BR-14800900 São Paulo, Brazil
