Author(s): Vinhote, J. F. C. ; Torres, A. F. C. ; Dantas, R. T. ; Praciano, T. P. ; Menezes, R. R. P. P. B. ; Sousa, D. F. ; Brito, T. S. ; Lima, F. J. B. ; Toyama, M. H. [UNESP] ; Magalhaes, P. J. ; Monteiro, H. S. A. ; Martins-Nunes, A. M. C.
Date: 2014
Persistent ID: http://hdl.handle.net/11449/347
Origin: Oasisbr
Subject(s): Polybia paulista; venom; kidney; aorta; MDCK
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP)
In the present study, the effects of Polybia paulista venom (PPV) on renal and vascular tissues were investigated. Isolated kidneys perfused with PPV (1 and 3 mu g/mL) had increased perfusion pressure, renal vascular resistance, urinary flow, and glomerular filtration rate; and reduced sodium tubular transport. Histological evaluation demonstrated deposits of proteins in Bowman's space and tubular lumen, and focal areas of necrosis. The venom promoted a cytotoxic effect on Madin-Darby canine kidney (MDCK) cells. A significant increase in lactic dehydrogenase levels was observed in response to venom exposure. In isolated mesenteric vascular beds, pressure and vascular resistance augmented in a dose-dependent manner. PPV increased the contractility of aortic rings maintained under basal tension. This contractile response was inhibited when preparations were maintained in Ca2+-free medium. Likewise, verapamil, a voltage-gated calcium channel blocker, also inhibited the contractile response. In this study, phentolamine, a blocker of a-adrenergic receptor blocker, significantly reduced the contractile effect of PPV in the aortic ring. In conclusion, PPV produced nephrotoxicity, which suggests a direct effect on necrotic cellular death in renal tubule cells. The vascular contractile effect of PPV appears to involve calcium influx through voltage-gated calcium channels via adrenergic regulation.
Universidade Federal do Ceará (UFC), Dept Clin & Toxicol Anal, BR-60420970 Fortaleza, Ceara, Brazil
Universidade Federal do Ceará (UFC), Dept Physiol & Pharmacol, BR-60420970 Fortaleza, Ceara, Brazil
UNESP Univ Estadual Paulista, São Paulo State Univ, Sao Vicente, SP, Brazil
UNESP Univ Estadual Paulista, São Paulo State Univ, Sao Vicente, SP, Brazil
