Autor(es): Lisboa, Lvia Aires ; Andrade, Evelyn Rabelo ; Esper, Cesar Roberto [UNESP] ; Seneda, Marcelo Marcondes
Data: 2014
Identificador Persistente: http://hdl.handle.net/11449/42621
Origem: Oasisbr
Assunto(s): Follicle size; OPU; Electron microscopy
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In vitro embryo production is an important technique for facilitating the reproduction of animals with high genetic merit. The greatest challenge for the reproducibility of this technique is the quality of the oocyte that is submitted for in vitro maturation. The aim of this work was to evaluate the ultrastructural characteristics of oocytes from follicles of different diameters using transmission electron microscopy. The animals were divided into 2 groups and were given a single i.m. injection of 250 IU FSH (Pluset, Serono, Italy). To synchronize the follicular growth, all follicles > 2 mm were aspirated during the estrous cycle, which was considered day zero (D0). Group 1 (G1; n = 4) received FSH on day 1 (D1), and the 2- to 5-mm follicles were aspirated on day 2 (D2). The animals in group 2 (G2; n = 5) received FSH on day 2 (D2), and their 10- to 15-mm follicles were aspirated on day 5 (D5). After aspiration, the oocytes from both groups were fixed and prepared for ultrastructural analysis. The oocytes analyzed from both groups had similar ultrastructural characteristics. The presence and distribution of organelles in the cytoplasm of the oocytes did not differ between groups, suggesting that, in relation to the ultrastructural characteristics, oocytes from 2 to 5 mm and 10 to 15 mm follicles are similar.
Universidade Estadual de Londrina (UEL), UEL, Londrina, PR USA
Univ Estadual Paulista, UNESP, Dept Med Vet Prevent & Reprod Anim, São Paulo, Brazil
UEL, Ctr Ciencias Agr, Dept Clin Vet, Londrina, PR USA
Univ Estadual Paulista, UNESP, Dept Med Vet Prevent & Reprod Anim, São Paulo, Brazil
