Simultaneous determination of Cd and Pb in antibiotics used in sugar-cane fermentation process by GFAAS

• Author(s): Amorim Filho, V. R. ; Polito, W. L. ; Oliveira, S. R. [UNESP] ; Freschi, G. P G [UNESP] ; Gomes Neto, José Anchieta [UNESP]
• Date: 2014
• Persistent ID: http://hdl.handle.net/11449/69087
• Origin: Oasisbr
• Subject(s): Antibiotic; Cadmium; GFAAS; Lead; Permanent modifier
• Document Type: Journal article
• Language: English

Description

Submitted by Vitor Silverio Rodrigues ([e-mail]) on 2014-05-27T11:21:58Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:34:49Z : No. of bitstreams: 1 2-s2.0-33748358911.pdf: 289778 bytes, checksum: fd63fa95eb61b390ad3176bd41fef936 (MD5)

Made available in DSpace on 2014-05-27T11:21:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-09-12

A method has been developed for the simultaneous determination of Cd and Pb in antibiotics used in sugar-cane fermentation by GFAAS. The integrated platform of transversely heated graphite atomizer was treated with tungsten to form a coating of tungsten carbide. Six samples of commercial solid antibiotics were analyzed by injecting 20 μL of digested samples into the pretreated graphite platform with co-injection of 5 μL of 1000 mg L-1 Pd as chemical modifier. Samples were mineralized in a closed-vessel microwave-assisted acid-digestion system using nitric acid plus hydrogen peroxide. The pyrolysis and atomization temperatures of the heating program of the atomizer were selected as 600°C and 2200°C, respectively. The calculated characteristic mass for Cd and Pb was 1.6 pg and 42 pg, respectively. Limits of detection (LOD) based on integrated absorbance were 0.02 μg L -1 Cd and 0.7 μg L-1 Pb and the relative standard deviations (n = 10) for Cd and Pb were 5.7% and 8.0%, respectively. The recoveries of Cd and Pb added to the digested samples varied from 91% to 125% (Cd) and 80% to 112% (Pb).

Instituto de Química de São Carlos Universidade de São Paulo, P.O. Box 780, 13560-970 São Carlos - SP

Departamento de Química Analítica Instituto de Química Universidade Estadual Paulista, P.O. Box 355, 14801-970 Araraquara - SP

Departamento de Química Analítica Instituto de Química Universidade Estadual Paulista, P.O. Box 355, 14801-970 Araraquara - SP