Author(s):
Botosso, Viviane F. ; Zanotto, Paolo M. de A. ; Ueda, Mirthes ; Arruda, Eurico ; Gilio, Alfredo E. ; Ieira, Sandra E. ; Stewien, Klaus E. ; Peret, Teresa C. T. ; Jamal, Leda F. ; Pardini, Maria I. de M. C. ; Pinho, Joãr ; Massad, Duardo ; Sant'Anna, Osvaldo A. ; Holmes, Eddie C. ; Durigon, Edison L. ; Comone, Priscila ; Do Sacramento, Patrícia R. ; Durigan, Mariana S. ; Oliveira, Danielle B. L. ; Moraes, Claudia T. P. ; Campo, Angélica C. A. ; Leal, Andréia L. ; Silva, Tereza S. ; Carvalho, Ariane C. L. ; Tenório, Elisabeth C. N. ; Cintra, Otavio A. L. ; Ansarah-Sobrinho, Camilo ; Proençna-Modena, José L. ; Iwamoto, Marisa A. ; De Paula, Flávia E. ; Souza, Maria C. O. ; Vaz-de-Lima, Lourdes R. A. ; Matsumoto, Tokiko K. ; Sato, Neuza N. ; Salgado, Maristela M. ; Hong, Marisa A. ; Requejo, Henry I. ; Barbosa, Maria L. ; Oliveiveira, Carmem A. F. ; Passos, Saulo D. ; Pecchini, Rogério ; Berezin, Eitan ; Schvartsman, Claudio ; Pannuti, Cláudio S. ; Candeias, João M. G. ; Han, Sang W. ; Garcia, José F. ; Carrilho, Flair J. ; Figueiredo, Luíz T. M. ; Duarte, Alberto J. Da S. ; Wolff, José L. C. ; Rahal, Paula [UNESP] ; Richtzenhain, Leonardo J. ; Gonçales Jr., Fernando L. ; De Lima, Edimo G.
Date: 2014
Persistent ID: http://hdl.handle.net/11449/70883
Origin: Oasisbr
Subject(s): epitope; guanine nucleotide binding protein; virus protein; amino acid substitution; child; codon; controlled study; gene sequence; genotype; human; infant; major clinical study; newborn; nonhuman; nucleotide sequence; phylogeny; preschool child; Respiratory syncytial pneumovirus; respiratory syncytial pneumovirus a; respiratory syncytial pneumovirus b; unindexed sequence; virus gene; genetic variability; genetics; molecular evolution; respiratory tract infection; Human respiratory syncytial virus; Hydrangea ringspot virus; Amino Acid Substitution; Epitopes; Evolution, Molecular; Genetic Variation; Genotype; GTP-Binding Proteins; Humans; Phylogeny; Respiratory Syncytial Viruses; Respiratory Tract Infections; Viral Proteins
Description
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Human respiratory syncytial virus (HRSV) is the major cause of lower respiratory tract infections in children under 5 years of age and the elderly, causing annual disease outbreaks during the fall and winter. Multiple lineages of the HRSVA and HRSVB serotypes co-circulate within a single outbreak and display a strongly temporal pattern of genetic variation, with a replacement of dominant genotypes occurring during consecutive years. In the present study we utilized phylogenetic methods to detect and map sites subject to adaptive evolution in the G protein of HRSVA and HRSVB. A total of 29 and 23 amino acid sites were found to be putatively positively selected in HRSVA and HRSVB, respectively. Several of these sites defined genotypes and lineages within genotypes in both groups, and correlated well with epitopes previously described in group A. Remarkably, 18 of these positively selected tended to revert in time to a previous codon state, producing a flipflop phylogenetic pattern. Such frequent evolutionary reversals in HRSV are indicative of a combination of frequent positive selection, reflecting the changing immune status of the human population, and a limited repertoire of functionally viable amino acids at specific amino acid sites.
Butantan Institute Virology Branch, Butantã, São Paulo
Department of Microbiology Institute of Biomedical Sciences University of São Paulo, São Paulo
Division of Medical Biology Adolfo Lutz Institute, São Paulo
Department of Cell Biology School of Medicine of Ribeirão Preto University of São Paulo, Ribeirão Preto, São Paulo
Pediatric Division University Hospital University of São Paulo, São Paulo
Division of Viral Diseases National Center for Immunization and Respiratory Diseases Centers for Disease Control and Prevention, Atlanta, GA
STD/AIDS Reference and Training Centre, São Paulo, São Paulo
State University of São Paulo, São Paulo
Tropical Medicine Institute University of São Paulo, São Paulo
Department of Legal Medicine University of São Paulo Medical School, São Paulo
Center for Infectious Disease Dynamics Department of Biology Pennsylvania State University, University Park, PA
Fogarty International Center National Institutes of Health, Bethesda, MD
