Author(s):
Souza-Moreira, Tatiana M. [UNESP] ; Moreira, Raquel Regina Duarte [UNESP] ; Sacramento, Luis V. S. [UNESP] ; Pietro, Rosemeire C. L. R. [UNESP]
Date: 2014
Persistent ID: http://hdl.handle.net/11449/71513
Origin: Oasisbr
Subject(s): Antimicrobial activity; Antioxidant activity; Chemical screening; Plinia cauliflora; 1,1 diphenyl 2 picrylhydrazyl; acetone; alcohol; chloroform; flavonoid; lipid; phenol; plant extract; Plinia cauliflora extract; scavenger; tannin derivative; unclassified drug; water; agar diffusion; antimicrobial activity; antioxidant activity; biological activity; Candida; controlled study; drug activity; drug screening; Gram negative bacterium; Gram positive bacterium; histochemistry; minimum inhibitory concentration; Myrtaceae; nonhuman; phytochemistry; plant leaf; spectrophotometry; standardization; thin layer chromatography
Description
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Programa de Apoio ao Desenvolvimento Científico da Faculdade de Ciências Farmacêuticas da UNESP (PADC)
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
In this work, chemical and biological activities of crude extracts obtained with 50% ethanol, 70% ethanol, acetone:water (7:3; v/v) and chloroform of Plinia cauliflora (DC.) Kausel, Myrtaceae, leaves, a native tree from several regions of Brazil, was investigated. Histochemical and phytochemical screenings were done according to characterization reactions and thin layer chromatography. To assist in extracts standardization, total phenol and flavonoids content spectrophotometric was performed. Antioxidant activity was analyzed by percentage of radical scavenging using DPPH solution. Antimicrobial activity was evaluated against Gram-positive, Gram-negative pathogenic bacteria and species of Candida using agar diffusion and minimal inhibitory concentration (MIC) determination methods according to standard methods. The leaves presented lipids at secretory cavity and phenols, mainly tannins, in nervures and palisade parenchyma. Polar extracts showed flavonoids, tannins and high content of phenols and flavonoids. The extracts showed great antioxidant activity and antimicrobial activity was better against Candida species than against bacteria.
Departamento de Fármacos e Medicamentos Pós-graduação em Ciências Farmacêuticas Faculdade de Ciências Farmaceuticas, Universidade Estadual Paulista 'Julio de Mesquita Filho', Rodovia Araraquara-Jaú, km 1, 14801-902 Araraquara-SP
Departamento de Princípios Ativos Naturais e Toxicologia Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista Júlio de Mesquita Filho, Rodovia Araraquara-Jaú, km 1, 14801-902 Araraquara-SP
Departamento de Fármacos e Medicamentos Pós-graduação em Ciências Farmacêuticas Faculdade de Ciências Farmaceuticas, Universidade Estadual Paulista 'Julio de Mesquita Filho', Rodovia Araraquara-Jaú, km 1, 14801-902 Araraquara-SP
Departamento de Princípios Ativos Naturais e Toxicologia Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista Júlio de Mesquita Filho, Rodovia Araraquara-Jaú, km 1, 14801-902 Araraquara-SP
