Autor(es): de Moraes, Julieta Rodini Engrácia [UNESP] ; Bozzo, Fabiana Rizzi [UNESP] ; Ozorio, Rodrigo O. A. ; Engrácia Filho, Jair Rodin [UNESP] ; de Moraes, Flávio Ruas [UNESP]
Data: 2014
Identificador Persistente: http://hdl.handle.net/11449/74021
Origem: Oasisbr
Assunto(s): Blood; Erythrocytes; Inflammation; Leucocytes; Thrombocytes
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The effects of swim bladder injection with thioglycolate, Escherichia coli lipopolysaccharide (LPS) and heat-inactivated Aeromonas hydrophila were assessed on hematological responses in pacu, Piaractus mesopotamicus (Characidae). A quantitative assessment was done on erythrocytes, thrombocytes e leucocytes at 6, 24, and 48 h pos-injection of the inflammatory agents and compared with fish injected with saline solution (control). Fish injected with inactivated A. hydrophila showed a reduction of erythrocytes and hemoglobin, whereas the hematocrit increased 6 h pos-injection. The results show that thioglycolate and LPS also induced a reduction on hemoglobin and an increase on the hematocrit. The thrombocytes count decreased 6 h post A. hydrophila injection, whereas increased 48 hours post LPS injection. The leukocytes count increased after 6 h post A. hydrophila injection, while the lymphocytes and PAS-positive granular leukocytes (PAS-LG) count decreased after 24 h post injection. In fish injected with thioglycolate or with LPS showed an increase in the LG-PAS counts when compared to A. hydrophila or control groups. The monocytes count was not affected by the different inflammatory agents.
Department of Veterinary Pathology São Paulo State University, Jaboticabal - SP
Centro Interdisciplinar de Investigação Marinha e Ambiental Universidade do Porto, Porto
Department of Veterinary Pathology São Paulo State University, Jaboticabal - SP
