Author(s):
Moreno, Ricardo ; Delgado, Pamela O ; Coelho, Patrícia G ; Marsicano, Sarah R ; Boas, Viviane Av ; Azzalis, Ligia A ; Junqueira, Virgínia Bc ; Rocha, Katya C ; De Abreu, Luiz Carlos ; Valenti, Vitor E [UNESP] ; Drezzet, Jefferson ; Pereira, Edimar Cristiano ; Fonseca, Fernando La
Date: 2014
Persistent ID: http://hdl.handle.net/11449/74387
Origin: Oasisbr
Subject(s): DNA; Nanotechnology; Neoplams; Prostate; blood level; blood sampling; cancer patient; centrifugation; DNA determination; DNA extraction; human; major clinical study; male; nanotechnology; prostate cancer; reliability; spectrophotometry
Description
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Background: Several studies seek biological markers that give diagnostic and degree of tumor development. The aim of this study was to validate the determination of plasma DNA using nanotechnology (Nanovue™-NV) in samples of 80 patients with prostate cancer. Methods. Blood samples of 80 patients of the Urology Ambulatory of Faculdade de Medicina do ABC with prostate cancer confirmed by anatomical-pathology criteria were analyzed. DNA extraction was performed using a GFX TM kit (Amersham Pharmacia Biotech, Inc, USA) following the adapted protocol. Plasma was subjected to centrifugation. Results: There was a big difference between the first and the second value obtained by NanoVue Only two samples had no differences between duplicates. Maximum difference between duplicates was 38 μg/mL. Average variation between 51 samples was 10.29 μg/mL, although 21 samples had differences above this average. No correlation was observed between pDNA obtained by traditional spectrophotometry and by nanotechnology. Conclusion: Determination of plasma DNA by nanotechnology was not reproducible. © 2013 Moreno et al; licensee BioMed Central Ltd.
Laboratório de Análises Clínicas Faculdade de Medicina Do ABC, Av. Príncipe de Gales 821, Santo André, CEP: 09060-650
Departamento de Ciências Biológicas Universidade Federal de São Paulo UNIFESP, Rua Prof. Artur Riedel, 275, Diadema, São Paulo 09972-270
Laboratório de Delineamento de Estudos e Escrita Científica Departamento de Morfologia e Fisiologia Faculdade de Medicina Do ABC, Santo André, Av. Príncipe de Gales, n. 821, Santo André, CEP: 09060-650
Programa de Pós-Graduação em Fisioterapia Faculdade de Ciências e Tecnologia Universidade Estadual Paulista, Rua Roberto Simonsen, 305, Presidente Prudente, São Paulo 19060-900
Programa de Pós-Graduação em Fisioterapia Faculdade de Ciências e Tecnologia Universidade Estadual Paulista, Rua Roberto Simonsen, 305, Presidente Prudente, São Paulo 19060-900
