Author(s):
Bonacorsi, Cibele ; Da Fonseca, Luiz Marcos [UNESP] ; Raddi, Maria Stella Gonçalves [UNESP] ; Kitagawa, Rodrigo Rezende ; Vilegas, Wagner [UNESP]
Date: 2014
Persistent ID: http://hdl.handle.net/11449/76294
Origin: Oasisbr
Subject(s): Alchornea glandulosa extract; Alchornea triplinervia extract; Anacardium humile extract; antioxidant; Byrsonima basiloba extract; Byrsonima intermedia extract; Davilla elliptica extract; Mouriri pusa extract; plant extract; Qualea grandiflora extract; Qualea multiflora extract; Qualea parviflora extract; unclassified drug; Alchornea glandulosa; Alchornea triplinervia; Anacardium humile; animal cell; animal experiment; animal model; antioxidant activity; bacterium culture; Brazil; Byrsonima basiloba; Byrsonima intermedia; cell viability; chemoluminescence; controlled study; Davilla elliptica; gastritis; Helicobacter pylori; male; medicinal plant; Mouriri pusa; neutrophil; nonhuman; peritoneum exudate; priority journal; Qualea grandiflora; Qualea multiflora; Qualea parviflora; rat; respiratory burst; stomach ulcer
Description
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Ten Brazilian medicinal plants used to treat gastritis and ulcers were carefully selected on the basis of ethnopharmacological importance and antiulcerogenic activity previously described. The antioxidant activity of the methanolic extracts was determined in analysis conditions that simulate a real biological activity on inhibition of the oxidative burst induced in neutrophils using Helicobacter pylori as activator, by a luminol-amplified chemiluminescence assay. The extracts, at low concentration (5 g/mL), exhibited a large variation in inhibitory effects of H. pylori-induced oxidative burst ranging from 48% inhibition to inactive, but all extracts, excluding Byrsonima intermedia, had inhibitory activity over 80% at the concentration of 100 g/mL. The total suppressive antioxidant capacity measured as the effective concentration, which represents the extract concentration producing 50% inhibition of the chemiluminescence induced by H. pylori, varies from 27.2 to 56.8 g/mL and was in the following order: Qualea parviflora > Qualea multiflora > Alchornea triplinervia > Qualea grandiflora > Anacardium humile > Davilla elliptica > Mouriri pusa > Byrsonima basiloba > Alchornea glandulosa > Byrsonima intermedia. The main groups of compounds in tested extracts are presented. Differences in the phytochemical profile, quantitatively and qualitatively, of these plants can explain and justify their protective effect on the gastric mucosa caused by the neutrophil-generated ROS that occurs when H. pylori displays its evasion mechanisms. © 2013 Cibele Bonacorsi et al.
Instituto de Ciências da Saúde Universidade Federal de Mato Grosso, 78557-267 Sinop, MT
Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista (UNESP), 14801-902 Araraquara, SP
Departamento de Ciências Farmacêuticas Universidade Federal Do Espírito Santo, 29040-090 Vitória, ES
Campus Experimental Do Litoral Paulista Universidade Estadual Paulista (UNESP), 11330-900 São Vicente, SP
Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista (UNESP), 14801-902 Araraquara, SP
Campus Experimental Do Litoral Paulista Universidade Estadual Paulista (UNESP), 11330-900 São Vicente, SP
