Author(s): Almeida, Carolina R. ; Ojopi, Elida P. ; Nunes, Caris M. ; Machado, Luis R. ; Takayanagui, Osvaldo M. ; Livramento, Jose A. ; Abraham, Ronaldo ; Gattaz, Wagner F. ; Vaz, Adelaide J. ; Dias-Neto, Emmanuel
Date: 2019
Origin: Oasisbr
Subject(s): Pcr; Csf; Neurocysticercosis; Taenia Solium; Liquor; United-States; Epilepsy; Clinical Neurology; Psychiatry; Neurosciences & Neurology; Psychiatry
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Neurocysticercosis is the most frequent parasitic infection of the CNS and the main cause of acquired epilepsy worldwide. Seizures are the most common symptoms of the disease, together with headache, involuntary movements, psychosis and a global mental deterioration. Absolute diagnostic criteria include the identification of cysticerci, with scolex, in the brain by MRI imaging. We demonstrate here, for the first time, that T. solium DNA is present in the cerebrospinal fluid of patients. The PCR amplification of the parasite DNA in the CSF enabled the correct identification of 29/30 cases (96.7 %). The PCR diagnosis of parasite DNA in the CSF may be a strong support for the diagnosis of neurocysticercosis.
Univ Sao Paulo, Fac Med, Inst Psiquiatria, Lab Neurociencias, BR-05403010 Sao Paulo, Brazil; Univ Sao Paulo, Fac Med, Dept Psiquiatria, BR-05403010 Sao Paulo, Brazil; UNESP, Dept Apoio Prod & Saude Anim, Aracatuba, SP, Brazil; Univ Sao Paulo, FMUSP, Dept Neurol, BR-05403010 Sao Paulo, Brazil; Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Neurol, BR-14048900 Ribeirao Preto, SP, Brazil; Universidade de Taubaté (Unitau), Dept Med; USP, Fac Ciencias Farmaceut, Lab Imunol Clin, BR-09500900 Sao Paulo, Brazil
