Author(s):
Mesquita, Vanessa A. ; Machado, Manuela D. ; Silva, Cristina F. ; Soares, Eduardo V.
Date: 2016
Persistent ID: http://hdl.handle.net/1822/43000
Origin: RepositóriUM - Universidade do Minho
Project/scholarship:
info:eu-repo/grantAgreement/FCT/5876/147337/PT;
info:eu-repo/grantAgreement/FCT/5876-PPCDTI/126270/PT
;
info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F72816%2F2010/PT;
Subject(s): 2-deoxy-d-glucose (2-DOG); Heavy metals toxicity; Membrane integrity; Sodium azide; Yeast viability; Science & Technology
Description
This work aims to examine the influence of the metabolic state of the yeast Pichia kudriavzevii on the susceptibility to a metals mixture (5mgL1 Cd, 10mgL1 Pb, and 5mgL1 Zn). Cells exposed to the metals mixture in the presence of 25mmolL1 glucose displayed a higher loss of membrane integrity and proliferation capacity, compared to cells incubated in the absence of glucose. The analysis of the effect of individual metals revealed that glucose increased the toxic effect of Cd marginally, and of Pb significantly. The increased susceptibility to heavy metals due to glucose was attenuated in the simultaneous presence of a mitochondrial respiration inhibitor such as sodium azide (NaN3). ATP-depleted yeast cells, resulting from treatment with the non-metabolizable glucose analogue 2-deoxy-d-glucose, showed an increased susceptibility to heavy metals mixture. Pre-incubation of yeast cells with 1 or 1.5mmolL1 Ca2+ reduced significantly (P<0.05) the loss of membrane integrity induced by the metals mixture. These findings contribute to the understanding of metals mechanisms of toxicity in the non-conventional yeast P. kudriavzevii.
This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and the Project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER- 027462). Vanessa A. Mesquita and Manuela D. Machado gratefully acknowledge the grant from Coordenaç~ao de Aperfeiçoamento de Pessoal de N ıvel Superior (CAPES) and the post-doctoral grant from FCT (SRH/BPD/72816/ 2010), respectively.
