Author(s):
Alves, M. J. ; Grenho, L. ; Lopes, Cláudia Jesus Ribeiro ; Borges, Joel Nuno Pinto ; Vaz, F. ; Vaz, I. P. ; Fernandes, M. H.
Date: 2018
Persistent ID: https://hdl.handle.net/1822/55880
Origin: RepositóriUM - Universidade do Minho
Project/scholarship:
info:eu-repo/grantAgreement/FCT/5876/147218/PT;
info:eu-repo/grantAgreement/FCT/5876/147414/PT;
info:eu-repo/grantAgreement/FCT/5876/147414/PT;
Subject(s): Antibacterial activity; Ar-plasma treatment; Cytocompatibility; Gutta-percha cones; UV activation; ZnO thin films; Science & Technology
Description
This work explored a novel approach to enhance the antibacterial activity of commercial Gutta-percha (GP) cones, the most commonly used core filling materials used in endodontic treatment. The reported procedure involved an argon (Ar) plasma treatment (PT) of the GP cone surface, followed by the deposition of a ZnO thin film by magnetron sputtering. The resulting surfaces were evaluated for surface topography, antibacterial activity against Enterococcus faecalis and Staphylococcus aureus, and cytocompatibility with human osteoblastic cells. GP cones treated with NaOCl, a routine chair-side protocol, were also tested as reference. The deposition of a ZnO film on pristine GP cones increased its antibacterial activity. Cones pre-treated with Ar-plasma (PT) and coated with the ZnO thin film presented significantly higher antibacterial activity than that observed on the pristine and, also, compared to the ZnO coated cones. The higher antibacterial activity of PT + ZnO cones appears related to the major effects induced by the PT pre-treatment on the cone surface endowing the deposited ZnO film with a homogeneous nanostructured topography that greatly improved surface reactivity. The modified GP cones maintained an appropriate cytocompatibility with human cells. This novel approach provides ready-to-use cones with enhanced antibacterial activity, improving a strict asepsis protocol during endodontic treatment and preventing secondary endodontic infections.
Financial support from the European Union (FEDER funds POCI/01/0145/FEDER/007265) and National Funds (FCT/MEC, Fundação para a Ciência e Tecnologia and Ministério da Educação e Ciência) under the Partnership Agreement PT2020 UID/QUI/50006/2013 is acknowledged. This research was also sponsored by the Portuguese Foundation for Science and Technology (FCT) in the framework of the Strategic Funding UID/FIS/04650/2013 and FCT Project 9471 - RIDTI, co-funded FEDER and with reference PTDC/FIS-NAN/1154/2014. J. Borges acknowledges FCT for his Postdoc Grant (SFRH/BPD/117010/2016) and C. Lopes acknowledges FCT for his PhD Grant (SFRH/BD/103373/2014).
info:eu-repo/semantics/publishedVersion
