Autor(es): González, Abigail ; Gonçalves, Clarisse Salomé Nobre ; Simões, Lívia S ; Cruz, Mario ; Loredo, Araceli ; Rodríguez, Rosa ; Contreras, Juan ; Teixeira, J. A. ; Belmares, Ruth
Data: 2021
Identificador Persistente: https://hdl.handle.net/1822/70931
Origem: RepositóriUM - Universidade do Minho
Assunto(s): Protein concentrate; Pleurotus ostreatus; edible mushroom; digestion; hydrolysis degree; antioxidant activity; In vitro digestion; Science & Technology
Edible mushrooms used as a protein-rich food may be an attractive alternative to conventional protein sources, while promoting its valorization. This work aimed to obtain a protein concentrate from a Pleurotus ostreatus mushroom flour, its characterization, and nutritional and functional properties evaluation. Methodologies applied for extraction and precipitation of protein were optimized - pH 4 and 12, respectively; and flour-solvent ratio of 1:20 w/v. The protein density was increased by 78 %. P. ostreatus flour and concentrate were characterized by proximal composition. The content of total phenolic compounds in the protein concentrate decreased, leading to a positive effect on protein digestibility, while the DPPH radical scavenging activity was not significantly affected. Peptides with molecular weights from 12 to 35 kDa, with possible bioactivity, were identified by electrophoresis. Protein digestibility assessed by in vitro gastrointestinal digestion showed a 4.2-fold higher hydrolysis degree in the protein concentrate than the flour.
This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation (NORTE–01–0145–FEDER-000004) funded by the European Regional Development Fund under the scope of Norte 2020–Programa Operacional Regional do Norte and the Project ColOsH PTDC/BTM–SAL/30071/2017 (POCI–01–0145–FEDER–030071). The authors also acknowledge the grant for a postgraduate scholarship (CVU number 922722) provided by National Council of Science and Technology (CONACYT) Mexico.
info:eu-repo/semantics/publishedVersion
