Author(s):
Ibáñez-Redín, Gisela ; Furuta, Roberto H. M. ; Wilson, Deivy ; Shimizu, Flavio M. ; Materon, Elsa M. ; Arantes, Lidia Maria Rebolho Batista ; Melendez, Matias E. ; Carvalho, André L. ; Reis, R. M. ; Chaur, Manuel N. ; Gonçalves, Débora ; Oliveira, Osvaldo N.
Date: 2019
Persistent ID: https://hdl.handle.net/1822/67287
Origin: RepositóriUM - Universidade do Minho
Subject(s): Antibodies; Biomarkers, Tumor; CA-19-9 Antigen; Carbon nano-onions; Electric Capacitance; Electrodes; Graphite; Humans; Nanostructures; Printing; Carbon nano-onions; Screen-printed electrodes; Capacitive biosensors; Low-cost sensors; CA19-9; Information visualization
Description
Nanostructured capacitive biosensors, combined with inexpensive fabrication technologies, may provide simple, sensitive devices for detecting clinically relevant cancer biomarkers. Herein, we report a novel platform for detecting the pancreatic cancer biomarker CA19-9 using low-cost screen-printed interdigitated electrodes (SPIDEs). The SPIDEs were modified by carbon nano-onions (CNOs) and graphene oxide (GO) films, on which a layer of anti-CA19-9 antibodies was immobilized. The modification with CNOs and GO significantly improved the analytical performance of the biosensor, which displayed superior results to those prepared only with GO. The biossensor exhibited high reproducibility and a relatively low limit of detection of 0.12 U mL-1. Using these devices in combination with information visualization methods we were able to detect CA19-9 in whole cell lysates of colorectal adenocarcinoma. The fabrication of these low-cost, disposable immunosensors is a successful attempt to explore CNOs in capacitive biosensors, which may be extended for detection of different cancer biomarkers.
