Author(s): Freitas, Ana Sofia ; Costa, Marta Sílvia Freitas ; Pontes, Olívia ; Seidel, Veronique ; Proença, M. Fernanda R. P. ; Cardoso, Susana M. ; Oliveira, Rui Pedro Soares de ; Baltazar, Fátima ; Almeida Aguiar, Cristina
Date: 2022
Persistent ID: https://hdl.handle.net/1822/80295
Origin: RepositóriUM - Universidade do Minho
Subject(s): Propolis; Fractionation; Phenolic compounds; Pectolinarigenin; Cytotoxic activity; Renal cell carcinoma
Renal cell carcinoma is the most lethal cancer of the urological system due to late diagnosis and treatment resistance. Propolis, a beehive product, is a valuable natural source of compounds with bioactivities and may be a beneficial addition to current anticancer treatments. A Portuguese propolis sample, its fractions (n-hexane, ethyl acetate, n-butanol and water) and three subfractions (P1–P3), were tested for their toxicity on A498, 786-O and Caki-2 renal cell carcinoma cell lines and the non-neoplastic HK2 kidney cells. The ethyl acetate fraction showed the strongest toxicity against A498 (IC50 = 0.162 µg mL−1) and 786-O (IC50 = 0.271 µg mL−1) cells. With similar toxicity against 786-O, P1 (IC50 = 3.8 µg mL−1) and P3 (IC50 = 3.1 µg mL−1) exhibited greater effect when combined (IC50 = 2.5 µg mL−1). Results support the potential of propolis and its constituents as promising coadjuvants in renal cell carcinoma treatment.
