Author(s): Azevedo, Maria A. ; Cerqueira, Miguel A. ; Gonçalves, Catarina ; Amado, Isabel R. ; Teixeira, José A. ; Pastrana, Lorenzo
Date: 2023
Persistent ID: https://hdl.handle.net/1822/85344
Origin: RepositóriUM - Universidade do Minho
Subject(s): Biosurfactant; Emulsification; Bioactive compounds; Nanotechnology; Nanoencapsulation
This work had as main objective to encapsulate vitamin D3 (VD3) into nanostructured lipid carriers (NLCs) using rhamnolipids as surfactant. Glycerol monostearate and medium chain triglycerides with 2.625 % of VD3 were used as lipid materials. The three formulations of NLCs with VD3 (NLCs + VD3) were composed by 99 % of aqueous phase, 1 % of lipid phase and 0.05 % of surfactant. The difference between them was the ratio of solid:liquid in lipid phase. The NLCs + VD3 sizes ranged between 92.1 and 108.1 nm. The most stable formulation maintaining their caracteristics for 60 days at 4 °C. The NLCs + VD3 cytotoxicity demonstrated that concentrations of 0.25 mg/mL or lower up had a good biocompatibility in vitro. During the in vitro digestion, formulations with lower sizes and higher content on solid lipid had higher lipolysis rate and consequently higher VD3 bioaccessibility. The rhamnolipids-based NLCs are a good option for the encapsulation of VD3.
