Author(s): Chen, Xinyi ; Yang, Xue ; Su, Xiaolei ; Cavaco-Paulo, Artur ; Su, Jing ; Wang, Hongbo
Date: 2024
Persistent ID: https://hdl.handle.net/1822/92880
Origin: RepositóriUM - Universidade do Minho
Subject(s): Laccase-like; Mn-F-Cu sheets; Self-assembly; Detection; Phenolic pollutants
Enzyme mimics with laccase-like activity showing excellent stability and tolerance have attracted extensive interest in phenolic pollutants treatment. Herein, we presented a mild and convenient strategy for the preparation of laccase-like sheets (denoted as Mn-F-Cu) inspired by the supramolecular self-assembly of the transition metals manganese, copper, and L-phenylalanine (F) under room temperature, alkaline conditions. The Mn-F-Cu sheets perform a better substrate affinity (Michaelis constant, Km = 0.65 mM) and a higher reaction rate (Vmax = 3.98 × 103 mM·min1) at 25 °C, pH 6.8 compared to natural laccase (Km = 1.08 mM; Vmax = 6.93 × 106 mM·min1). Besides, the catalytic stability data show they also exhibit good tolerance under alkaline pH (pH 810) and high temperature (7090 °C). More importantly, the prepared Mn-F-Cu material shows a relatively better sensitivity in detecting phenolic pollutants 2,4-DP and catechol than other laccase mimics mentioned in the literature, with a detection limit of 2.41 M and 3.49 M, respectively(R2 > 0.99). Therefore, Mn-F-Cu sheets show great potential to replace natural laccase for the detection and treatment of phenolic pollutants in harsh environments.
