Author(s): Chen, Xinyi ; Tao, Wenwen ; Xu, Ruiguang ; Mo, Taoling ; Yang, Xue ; Cavaco-Paulo, Artur ; Su, Jing ; Wang, Hongbo
Date: 2025
Persistent ID: https://hdl.handle.net/1822/96953
Origin: RepositóriUM - Universidade do Minho
Subject(s): Core-shell structure; Dye decolorization; Laccase-like enzyme; Phenolic pollutant detection
Laccase shows significant potential for environmental remediation, yet its practical implementation is limited by inherent instability and restricted reusability. To address these limitations, we successfully synthesized a Mn/Cu-based core-shell structured laccase-like enzyme (SiO2@APTCu-MnO2) using a sol-gel method coupled with an in situ sacrificial strategy. Compared to natural laccase, SiO2@APTCu-MnO2 exhibited enhanced substrate affinity (Michaelis constant, Km = 0.29 mM) and a higher catalytic rate (Vmax = 1.05 ×10³ mM/min). It maintained exceptional stability across wide pH and temperature ranges, retaining over 80 % activity after 90 days. During application, the decolorization efficiency for anthraquinone and azo dyes was over 90 % within 2 h. Remarkably, it preserved over 80 % of its activity and decolorization capacity for reactive blue 19 even after 10 cycles. A dye decolorization-cultivation system confirmed that the treated water had minimal impact on plant growth. Additionally, the laccase-like enzyme enabled rapid and sensitive detection of phenolic pollutants. These results indicate that SiO2@APTCu-MnO serves as a high-performance, multifunctional alternative to natural laccase in environmental remediation and pollutant detection.
