Glutamine treatment attenuates hyperglycemia-induced mitochondrial stress and apoptosis in umbilical vein endothelial cells

• Author(s): Safi, Sher Zaman ; Batumalaie, Kalaivani ; Mansor, Marzida ; Chinna, Karuthan ; Mohan, Syam ; Karimian, Hamed ; Qvist, Rajes ; Ashraf, Muhammad Aqeel ; Yan, Garcie Ong Siok
• Date: 2015
• Origin: Oasisbr
• Document Type: Journal article
• Language: English

Description

OBJECTIVE: The aim of this study was to determine the in vitro effect of glutamine and insulin on apoptosis, mitochondrial membrane potential, cell permeability, and inflammatory cytokines in hyperglycemic umbilical vein endothelial cells. MATERIALS AND METHODS: Human umbilical vein endothelial cells were grown and subjected to glutamine and insulin to examine the effects of these agents on the hyperglycemic state. Mitochondrial function and the production of inflammatory cytokines were assessed using fluorescence analysis and multiple cytotoxicity assays. Apoptosis was analyzed by the terminal deoxynucleotidyl transferase dUTP nick end-labeling assay. RESULTS: Glutamine maintains the integrity of the mitochondria by reducing the cell permeability and cytochrome c levels and increasing the mitochondrial membrane potential. The cytochrome c level was significantly (p