Author(s): Cubas, Jairo Jose Matozinho ; Simões, Ricardo Santos ; Oliveira-Filho, Ricardo Martins ; Simões, Manuel Jesus ; Baracat, Edmund C ; Soares-Jr, José Maria
Date: 2010
Origin: Oasisbr
Subject(s): Heparan sulfate; Dermatan sulfate; Hyaluronic acid; Cervix uteri; Estrous cycle
OBJECTIVE: To analyze the amount of glycosaminoglycans in the uterine cervix during each phase of the rat estrous cycle. DESIGN: Based on vaginal smears, forty female, regularly cycling rats were divided into four groups (n = 10 for each group): GI - proestrous, GII - estrous, GIII - metaestrous and GIV - diestrous. Animals were sacrificed at each phase of the cycle, and the cervix was immediately removed and submitted to biochemical extraction and determination of sulfated glycosaminoglycans and hyaluronic acid. The results were analyzed by ANOVA followed by the Bonferroni post-hoc test. RESULTS: The uterine cervix had the highest amount of total sulfated glycosaminoglycans and dermatan sulfate during the estrous phase (8.90 ± 0.55 mg/g of cetonic extract, p
