Autor(es):
Gomes, Inês ; Rodrigues, Helena ; Rodrigues, Carla ; Marques, Marta ; Paíga, Paula ; Paiva, Alexandre ; Simões, Pedro ; Fernandes, Virgínia Cruz ; Vieira, Mónica ; Delerue-Matos, Cristina ; Soares, Cristina ; Grosso, Clara
Data: 2022
Identificador Persistente: http://hdl.handle.net/10362/151086
Origem: Repositório Institucional da UNL
Projeto/bolsa:
info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FOCE-ETA%2F30240%2F2017/PT;
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT;
info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT;
info:eu-repo/grantAgreement/FCT/Investigador FCT/IF%2F01146%2F2015%2FCP1293%2FCT0003/PT;
info:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBPD%2F109153%2F2015/PT;
Assunto(s): Iodine; Maillard reaction; Neuroprotection; Nitrosative stress; Oxidative stress; Pesticides; Pharmaceuticals; Seaweeds; Total phenolic content; Food Science; Microbiology; Health(social science); Health Professions (miscellaneous); Plant Science
Descrição
This work was financed by FEDER—Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020—Operacional Programme for Competitiveness and Internationalisation (POCI). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Neuroprotection is a need that remains unmet in treating chronic neurodegenerative disorders, despite decades of extensive research. To find new neuroprotective compounds, extracts of Himanthalia elongata (L.) S.F.Gray and of Eisenia bicyclis (Kjellman) Setchell were obtained through subcritical water extraction applying a four-step temperature gradient. The fractions obtained were screened against brain enzymes involved in neurodegenerative etiology, namely in Alzheimer’s and Parkinson’s diseases, and against reactive oxygen and nitrogen species, all contributing factors to the progression of neurodegeneration. Results showed no significant enzyme inhibition but strong radical scavenging activities, particularly in the fourth fraction, extracted at the highest temperature (250 °C), highlighting their ability to retard oxidative and nitrosative stresses. At higher temperatures, fractions were composed of phenolic compounds and Maillard reaction products, a combination that contributed to their antioxidant activity and, consequently, their neuroprotective properties. All fractions were evaluated for the presence of iodine, 14 organochlorine and 7 organophosphorus pesticides, and pharmaceuticals used in Alzheimer’s and Parkinson’s diseases (14), psychiatric drugs (8), and metabolites (8). The fractions studied did not present any of the screened contaminants, and only fraction 1 of E. bicyclis should be used with caution due to iodine content.
